Len Pharma, Zamkowa 17, 82-200 Malbork, Poland.
Biotechnol Prog. 2012 Sep-Oct;28(5):1336-46. doi: 10.1002/btpr.1599. Epub 2012 Aug 17.
A new generation of entirely biodegradable and bioactive composites with polylactic acid (PLA) or poly-ε-caprolactone (PCL) as the matrix and bioplastic flax fibers as reinforcement were analyzed. Bioplastic fibers contain polyhydroxybutyrate and were obtained from transgenic flax. Biochemical analysis of fibers revealed presence of several antioxidative compounds of hydrophilic (phenolics) and hydrophobic [cannabidiol (CBD), lutein] nature, indicating their high antioxidant potential. The presence of CBD and lutein in flax fibers is reported for the first time. FTIR analysis showed intermolecular hydrogen bonds between the constituents in composite PLA+flax fibers which were not detected in PCL-based composite. Mechanical analysis of prepared composites revealed improved stiffness and a decrease in tensile strength. The viability of human dermal fibroblasts on the surface of composites made of PLA and transgenic flax fibers was the same as for cells cultured without composites and only slightly lower (to 9%) for PCL-based composites. The amount of platelets and Escherichia coli cells aggregated on the surface of the PLA based composites was significantly lower than for pure polymer. Thus, composites made of PLA and transgenic flax fibers seem to have bacteriostatic, platelet anti-aggregated, and non-cytotoxic effect.
分析了新一代完全可生物降解和生物活性的复合材料，其基体为聚乳酸（PLA）或聚己内酯（PCL），增强材料为生物塑料亚麻纤维。生物塑料纤维含有聚羟基丁酸酯，是从转基因亚麻中获得的。纤维的生化分析表明，存在几种亲水性（酚类）和疏水性（大麻二酚[CBD]、叶黄素）的抗氧化化合物，表明其具有很高的抗氧化潜力。这是首次报道 CBD 和叶黄素存在于亚麻纤维中。傅里叶变换红外（FTIR）分析表明，在 PLA+亚麻纤维复合材料的组成部分之间存在分子间氢键，而在基于 PCL 的复合材料中未检测到这些氢键。对制备的复合材料进行机械分析表明，其刚性提高，拉伸强度降低。在 PLA 和转基因亚麻纤维制成的复合材料表面上培养的人皮肤成纤维细胞的活力与未培养复合材料的细胞相同，而对于基于 PCL 的复合材料，其活力仅略低（低 9%）。在 PLA 基复合材料表面上聚集的血小板和大肠杆菌细胞数量明显低于纯聚合物。因此，由 PLA 和转基因亚麻纤维制成的复合材料似乎具有抑菌、抗血小板聚集和非细胞毒性作用。
