Wells R D, Hutchinson M A, Eckhart W
J Virol. 1979 Nov;32(2):517-22. doi: 10.1128/JVI.32.2.517-522.1979.
We introduced deletions in the early region of the polyoma virus genome near the HaeII restriction enzyme cleavage site, between the origin of viral DNA replication and the site of initiation of translation of the polyoma T antigens. We analyzed the DNA of the deletion mutants by restriction enzyme digestion. Four of the mutants had deletions beginning very close to the HaeII site and extending clockwise toward the site of initiation of translation. The deletions near the HaeII site varied in size from about 10 base pairs to about 55 base pairs. The mutants containing deletions near the HaeII site were capable of lytic growth in mouse 3T6 cells and were capable of transforming rat F2408 cells, as judged by focus formation.
我们在多瘤病毒基因组早期区域靠近HaeII限制性内切酶切割位点处引入缺失，该位点位于病毒DNA复制起点与多瘤T抗原翻译起始位点之间。我们通过限制性内切酶消化分析了缺失突变体的DNA。其中四个突变体的缺失从非常靠近HaeII位点处开始，并沿顺时针方向延伸至翻译起始位点。靠近HaeII位点的缺失大小在约10个碱基对至约55个碱基对之间变化。通过焦点形成判断，含有靠近HaeII位点缺失的突变体能够在小鼠3T6细胞中进行裂解生长，并能够转化大鼠F2408细胞。
