Pauley R J, Medina D, Socher S H
J Virol. 1979 Nov;32(2):557-66. doi: 10.1128/JVI.32.2.557-566.1979.
The effects of glucocorticoids and prolactin on murine mammary tumor virus (MuMTV) RNA expression in preneoplastic outgrowth lines and mammary tumors in BALB/c mice were investigated. Hyperplastic alveolar nodules (HAN) and a ductal hyperplasia (DH) are induced in virgin BALB/c mice by prolonged hormonal stimulation or treatment with 7,12-dimethylbenz(a)anthracene or both. Mice bearing HAN or DH outgrowth lines and mammary tumors that arose from the outgrowth lines were treated with glucocorticoids or prolactin. MuMTV RNA was quantitated by hybridization with a representative complementary DNA probe specific for MuMTV RNA. Prolactin treatment did not increase MuMTV RNA in the BALB/c HAN or DH outgrowth lines or tumors. MuMTV RNA increased after glucocorticoid treatment in the C3, C4, and C5 HAN outgrowth lines and in tumors that arose from the D1, D2, C4, and C5 HAN and CD8 DH outgrowth lines. No increase in MuMTV RNA with glucocorticoid treatment was observed in the D1 or D2 HAN outgrowth line, in the CD8 DH outgrowth lines, and in tumors that arose from the C3 HAN outgrowth line. The ability of glucocorticoids to stimulate MuMTV expression was specific since the response was dose dependent and specific for glucocorticoid hormones. Glucocorticoid treatment did not increase the level of type C viral RNA in the majority of hormone- or 7,12-dimethylbenz(a)anthracene-induced HAN outgrowth lines or tumors. These observations suggested that glucocorticoids may influence MuMTV expression during mammary tumorigenesis in BALB/c mice.
研究了糖皮质激素和催乳素对BALB/c小鼠肿瘤前生长系和乳腺肿瘤中鼠乳腺肿瘤病毒（MuMTV）RNA表达的影响。通过长期激素刺激或用7,12-二甲基苯并(a)蒽或两者处理，可在未交配的BALB/c小鼠中诱导出增生性肺泡结节（HAN）和导管增生（DH）。对携带HAN或DH生长系以及由这些生长系产生的乳腺肿瘤的小鼠给予糖皮质激素或催乳素处理。通过与特异性针对MuMTV RNA的代表性互补DNA探针杂交来定量MuMTV RNA。催乳素处理并未增加BALB/c HAN或DH生长系或肿瘤中的MuMTV RNA。在C3、C4和C5 HAN生长系以及由D1、D2、C4和C5 HAN以及CD8 DH生长系产生的肿瘤中，糖皮质激素处理后MuMTV RNA增加。在D1或D2 HAN生长系、CD8 DH生长系以及由C3 HAN生长系产生的肿瘤中，未观察到糖皮质激素处理后MuMTV RNA增加。糖皮质激素刺激MuMTV表达的能力具有特异性，因为这种反应是剂量依赖性的，且对糖皮质激素具有特异性。糖皮质激素处理并未增加大多数激素或7,12-二甲基苯并(a)蒽诱导的HAN生长系或肿瘤中C型病毒RNA的水平。这些观察结果表明糖皮质激素可能会影响BALB/c小鼠乳腺肿瘤发生过程中MuMTV的表达。
