Callahan R, Benveniste R E, Lieber M M, Todaro G J
J Virol. 1974 Dec;14(6):1394-403. doi: 10.1128/JVI.14.6.1394-1403.1974.
The nucleic acid sequence homology between various murine, endogenous type-C viruses (three host range classes of BALB/c virus, the AT-124 virus, and the CCL 52 virus) and two laboratory strains of murine leukemia virus (Rauscher and Kirsten) was determined by DNA:RNA hybridization. The viral sequences exhibit varying degrees of partial homology. DNA:DNA hybridizations were performed between [(3)H]DNA probes prepared from N- and X-tropic BALB/c endogenous viruses and cellular DNAs from BALB/c, NIH Swiss, and AKR inbred mouse strains as well as from California feral mice and the Asian mouse subspecies Mus musculus molossinus and M. musculus castaneus. All of these strains of mice are shown to possess multiple (six to seven per haploid genome), partially related copies of type-C virogenes in their DNAs. Thermal melting profiles of the DNA:RNA and DNA:DNA hybrids suggest that the partial homology of the viral nucleic acid sequences is the result of base alterations throughout the viral genomes, rather than the loss of discrete segments of viral sequences.
通过DNA:RNA杂交测定了各种鼠源内源性C型病毒（BALB/c病毒的三种宿主范围类别、AT - 124病毒和CCL 52病毒）与两种鼠白血病病毒实验室毒株（劳舍尔病毒和柯斯顿病毒）之间的核酸序列同源性。病毒序列呈现出不同程度的部分同源性。用从N - 嗜性和X - 嗜性BALB/c内源性病毒制备的[(3)H]DNA探针与BALB/c、NIH瑞士小鼠、AKR近交系小鼠品系以及加利福尼亚野生小鼠和亚洲小鼠亚种小家鼠和栗色小家鼠的细胞DNA进行了DNA:DNA杂交。所有这些小鼠品系在其DNA中均显示拥有多个（每个单倍体基因组六到七个）部分相关的C型病毒基因拷贝。DNA:RNA和DNA:DNA杂交体的热解链曲线表明，病毒核酸序列的部分同源性是整个病毒基因组碱基改变的结果,而非病毒序列离散片段的丢失。
