Cardiff R D, Morris D W, Young L J
J Natl Cancer Inst. 1983 Nov;71(5):1011-9.
The patterns of the milk-transmitted (exogenous) mouse mammary tumor virus (MuMTV) DNA restriction endonuclease fragments in the nodule and tumor stages of BALB/cfC3H mouse mammary neoplasia were compared with the use of the Southern blot analysis. Acquired MuMTV restriction fragments were detected in DNA from hyperplastic alveolar nodules (HAN), from primary hyperplastic outgrowths (HPO), from families of transplanted HPO, from tumors from HPO, and from serially transplanted tumors. The restriction fragment patterns suggested that the HAN were composed of clonal dominant populations. Transplantation of subdivisions of individual HAN resulted in HPO with DNA restriction patterns suggesting that HAN also contained two or more subpopulations. In all cases, HAN subpopulations shared MuMTV restriction fragments suggesting a common origin. Forty-seven tumors arising from HPO shared MuMTV restriction fragments with the HPO. Most but not all tumors had additional acquired MuMTV restriction fragments not detected in the progenitor HPO, indicating that they were composed of a distinct subpopulation that originated from the HPO. The restriction fragment pattern in some tumor lines was remarkably stable through many transplant generations. Some tumors had no major additional restriction fragments, suggesting that major rearrangements of MuMTV DNA are not required for tumorigenesis.
运用Southern印迹分析法，比较了BALB/cfC3H小鼠乳腺肿瘤形成的结节期和肿瘤期乳汁传播（外源性）小鼠乳腺肿瘤病毒（MuMTV）DNA限制性内切酶片段的模式。在增生性肺泡结节（HAN）、原发性增生性赘生物（HPO）、移植的HPO家族、HPO来源的肿瘤以及连续移植的肿瘤的DNA中，检测到了获得性MuMTV限制性片段。限制性片段模式表明，HAN由克隆优势群体组成。对单个HAN的细分进行移植，产生的HPO具有DNA限制性模式，表明HAN也包含两个或更多亚群。在所有情况下，HAN亚群共享MuMTV限制性片段，提示有共同起源。47个源自HPO的肿瘤与HPO共享MuMTV限制性片段。大多数但并非所有肿瘤都有在祖代HPO中未检测到的额外获得性MuMTV限制性片段，这表明它们由源自HPO的不同亚群组成。某些肿瘤系中的限制性片段模式在许多移植代中都非常稳定。一些肿瘤没有主要的额外限制性片段，这表明肿瘤发生不需要MuMTV DNA的主要重排。
