Kasamatsu H, Nehorayan A
J Virol. 1979 Nov;32(2):648-60. doi: 10.1128/JVI.32.2.648-660.1979.
African green monkey kidney cells infected by simian virus 40 were analyzed by immunofluorescence techniques for the nature and the time course of the appearance of viral polypeptides during infection. Reagents used in the study were anti-Vpl sera and affinity-purified anti-Vpl immunoglobulin G, anti-Vp3 sera, antivirus (anti-V) sera, and anti-tumor antigen sera. The results are summarized as follows. (i) Three types of staining, nuclear, perinuclear, and perinuclear accompanied by cytoplasmic staining, were observed in infected cells in reaction with anti-vpl antibody. In addition, a highly structured staining was observed at the periphery of nuclei of infected cells late in infection. (ii) In reaction with anti-Vp3 serum, the staining was confined within nuclei of cells throughout infection. (iii) Vp1 and Vp3 antigens seem to occupy different spacial regions of the nuclear area in cells. (iv) Vp1 and Vp3 antigens were expressed simultaneously during infection. (v) Centriolar staining observed early in infection paralleled the appearance of tumor (T-) antigen until 24 h after infection, after which time the frequency of positive centriolar staining decreased as infection progressed. (vi) T-antigen was first expressed at about 8 h after infection, and Vp1 and Vp3 antigens were first expressed at about 20 h after infection.
利用免疫荧光技术分析了感染猿猴病毒40的非洲绿猴肾细胞，以研究感染过程中病毒多肽出现的性质和时间进程。该研究中使用的试剂有抗Vp1血清、亲和纯化的抗Vp1免疫球蛋白G、抗Vp3血清、抗病毒（抗V）血清和抗肿瘤抗原血清。结果总结如下：（i）在与抗Vp1抗体反应时，感染细胞中观察到三种染色类型，即核染色、核周染色以及伴有胞质染色的核周染色。此外，在感染后期，感染细胞的细胞核周边观察到高度结构化的染色。（ii）在与抗Vp3血清反应时，整个感染过程中染色都局限在细胞核内。（iii）Vp1和Vp3抗原似乎占据细胞内核区的不同空间区域。（iv）Vp1和Vp3抗原在感染过程中同时表达。（v）感染早期观察到的中心粒染色与肿瘤（T-）抗原的出现平行，直到感染后24小时，此后随着感染进展，中心粒阳性染色的频率降低。（vi）T抗原在感染后约8小时首次表达，Vp1和Vp3抗原在感染后约20小时首次表达。
