Abdigoudarzi M, Esmaeilnia K, Shariat N
Reference Laboratory for Ticks and Tick Borne Diseases, Department of Parasitology, Razi Vaccine and Serum Research Institute, Karadj, Iran.
Iran J Arthropod Borne Dis. 2009;3(2):36-43. Epub 2009 Dec 31.
Chemical control method using different acaricides as spray, dipping solution or pour-on is routinely used for controlling ticks. Biological control agents are favorable due to their safety for animals and environment. Entomopathogenic fungi such as Beauveria bassiana are well known for controlling ticks. In this study, two Iranian indigenous strains of B. bassiana (B. bassiana 5197 and B. bassiana Evin) were selected and grown on specific media. The pathogenic effects of these strains were evaluated on adult stages of two Iranian Ixodidae members (H. anatolicum anatolicum Koch 1844, and H. marginatum Koch 1844) by dipping method.
Two Iranian strains of Beauveria bassiana (Beauveria bassiana 5197 and Beauveria bassiana Evin) were selected and were grown successfully on specific media. The pathogenic effects of these strains were evaluated on adult stages of Iranian Ixodidae members such as, Hyalomma anatolicum anatolicum and H. marginatum by dipping method (these ticks were grown up at laboratory conditions during 2002 up to 2003 and still it is continued) .
There was no effect of strain 5197 on mortality or fecundity rates for ticks. There was acute phase sign of paralysis in test group after dipping ticks in suspension made from Evin strain of B. bassiana. In addition, the test groups were totally died after four months, but the control groups survived for six months.
High concentration of fungal spores is needed for inducing fungal infection. Additional study using different strains and fungi on Iranian ticks is proposed.
使用不同杀螨剂进行喷雾、浸液或浇泼处理的化学防治方法常用于蜱虫防治。生物防治剂因其对动物和环境的安全性而备受青睐。球孢白僵菌等昆虫病原真菌以防治蜱虫而闻名。在本研究中，选择了两株伊朗本土的球孢白僵菌菌株（球孢白僵菌5197和球孢白僵菌埃温），并在特定培养基上培养。通过浸液法评估这些菌株对两种伊朗硬蜱科成员（安纳托利亚璃眼蜱指名亚种，1844年，科赫；边缘璃眼蜱，1844年，科赫）成虫阶段的致病效果。
选择两株伊朗的球孢白僵菌菌株（球孢白僵菌5197和球孢白僵菌埃温），并成功在特定培养基上培养。通过浸液法评估这些菌株对伊朗硬蜱科成员如安纳托利亚璃眼蜱和边缘璃眼蜱成虫阶段的致病效果（这些蜱虫于2002年至2003年在实验室条件下饲养，目前仍在继续）。
菌株5197对蜱虫的死亡率或繁殖率没有影响。在用球孢白僵菌埃温菌株制成的悬浮液浸蜱后，试验组出现了麻痹的急性期症状。此外，试验组在四个月后全部死亡，而对照组存活了六个月。
诱导真菌感染需要高浓度的真菌孢子。建议对伊朗蜱虫使用不同菌株和真菌进行进一步研究。
