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伊朗东南部绵羊和山羊蜱中伯氏考克斯氏体的聚合酶链反应检测

PCR-Detection of Coxiella burnetii in Ticks Collected from Sheep and Goats in Southeast Iran.

作者信息

Fard Sr Nourollahi, Khalili M

机构信息

Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.

出版信息

Iran J Arthropod Borne Dis. 2011;5(1):1-6. Epub 2011 Jun 30.

PMID:22808404
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3385568/
Abstract

BACKGROUND

There is a little data on Coxiella burnetii (Q fever agent) in Iran. Ticks may play a significant role in the transmission of C. burnetii among animals. The aim of this study was to use polymerase chain reaction for the detection of C. burnetii in ticks collected in Southeast Iran.

METHODS

One hundred and sixty ticks were collected from domestic animals in three localities of Kerman Province, Southeast Iran from November to June 2009. The collected ticks were divided into 35 pools and examined by Trans-PCR for C. burnetii.

RESULTS

Three pools, each consisting of five female of Hyalomma anatolicum anatolicum and one pool (6 ticks) of Rhipicephalus sanguineus ticks collected from goats and sheep were found to be positive by Trans-PCR.

CONCLUSION

This paper documents the first molecular detection of C. burnetii in ticks, which shows their role as putative vectors and reservoirs for this pathogenic agent.

摘要

背景

伊朗关于伯氏考克斯体(Q热病原体)的数据很少。蜱可能在动物间伯氏考克斯体的传播中起重要作用。本研究的目的是利用聚合酶链反应检测伊朗东南部采集的蜱中伯氏考克斯体。

方法

2009年11月至6月,从伊朗东南部克尔曼省三个地区的家畜身上采集了160只蜱。将采集的蜱分成35组,通过转录聚合酶链反应检测伯氏考克斯体。

结果

通过转录聚合酶链反应发现,有三组检测呈阳性,每组由五只安纳托利亚璃眼蜱雌蜱组成,还有一组(6只蜱)是从山羊和绵羊身上采集的血红扇头蜱。

结论

本文记录了首次在蜱中进行的伯氏考克斯体分子检测,表明蜱作为该病原体假定传播媒介和宿主的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da95/3385568/d03a1b3102d0/ijad-5-1f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da95/3385568/d03a1b3102d0/ijad-5-1f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da95/3385568/d03a1b3102d0/ijad-5-1f1.jpg

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J Med Microbiol. 2011 Jan;60(Pt 1):63-68. doi: 10.1099/jmm.0.021543-0. Epub 2010 Sep 30.
2
Q fever serology in febrile patients in southeast Iran.伊朗东南部发热患者的 Q 热血清学。
Trans R Soc Trop Med Hyg. 2010 Sep;104(9):623-4. doi: 10.1016/j.trstmh.2010.04.002. Epub 2010 Jun 2.
3
The first serologic study of Q fever in sheep in Iran.
使用贝叶斯潜在类别分析比较传统聚合酶链反应(PCR)和两种便于现场操作的检测方法在蜱虫中检测DNA的检测性能。
Front Vet Sci. 2024 Jun 19;11:1396714. doi: 10.3389/fvets.2024.1396714. eCollection 2024.
4
Molecular detection of Coxiella burnetii in tick and blood samples from small ruminants in northwest of Iran.伊朗西北部蜱和小反刍动物血液样本中贝氏柯克斯体的分子检测。
Exp Appl Acarol. 2024 Apr;92(3):529-546. doi: 10.1007/s10493-023-00888-y. Epub 2024 Feb 26.
5
The Molecular Detection of Bacterial Infections of Public Health Importance in Hard Tick () Nymphs Collected from the Forest Fringes of Western Ghats in the Goa, Karnataka and Maharashtra States of India.对从印度果阿邦、卡纳塔克邦和马哈拉施特拉邦西高止山脉森林边缘采集的硬蜱若虫中具有公共卫生重要性的细菌感染进行分子检测。
Microorganisms. 2023 Dec 28;12(1):52. doi: 10.3390/microorganisms12010052.
6
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4
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5
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8
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9
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10
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