Pavlov K A, Gurina O I, Antonova O M, Semenova A V, Chekhonin V P
Laboratory of Immunochemistry, Department of Biological Psychiatry, V. P. Serbskii Center of Social and Forensic Psychiatry, Russian State Medical University, Moscow, Russia.
Bull Exp Biol Med. 2011 Dec;152(2):206-9. doi: 10.1007/s10517-011-1489-3.
cDNA fragment encoding neuron-specific enolase was amplified from the cDNA library of human brain. Then the fragment was cloned for expression in E. coli using the vector pET28-a. High level of neuron-specific enolase expression was confirmed by SDS-PAAG electrophoresis and immunochemical identity by immunoblot analysis. The constructed producer strain is the cheapest source of neuron-specific enolase suitable for the use in diagnostic applications.
从人脑cDNA文库中扩增出编码神经元特异性烯醇化酶的cDNA片段。然后使用载体pET28-a将该片段克隆到大肠杆菌中进行表达。通过SDS-PAAG电泳确认了高水平的神经元特异性烯醇化酶表达，并通过免疫印迹分析确定了免疫化学特性。构建的生产菌株是适用于诊断应用的最便宜的神经元特异性烯醇化酶来源。
