Jackson J C, Lopes J M
Department of Molecular and Cellular Biochemistry, Loyola University of Chicago, Maywood, IL 60153.
Gene. 1995 Feb 27;154(1):109-13. doi: 10.1016/0378-1119(94)00877-u.
Here we report the isolation of an enolase (Eno)-encoding cDNA clone from Schizosaccharomyces pombe. The deduced amino acid (aa) sequence of the 1.4-kb cDNA shares identifies with a number of Eno from Escherichia coli to humans. The highest degree of similarity is to the known Eno from Saccharomyces cerevisiae and an Eno from Candida albicans. Northern blot analysis identified a single transcript of approx. 1.4 kb, which was most abundant when cells were grown in media with glucose as the carbon source, as opposed to glycerol/lactate or ethanol.
在此,我们报道了从粟酒裂殖酵母中分离出一个编码烯醇化酶(Eno)的cDNA克隆。该1.4 kb cDNA推导的氨基酸(aa)序列与从大肠杆菌到人类的多种烯醇化酶具有一致性。相似度最高的是酿酒酵母中已知的烯醇化酶和白色念珠菌中的一种烯醇化酶。Northern印迹分析鉴定出一条约1.4 kb的单一转录本,当细胞在以葡萄糖作为碳源的培养基中生长时,该转录本最为丰富,而在甘油/乳酸或乙醇培养基中生长时则不然。