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多瘤病毒颗粒和衣壳对小鼠肾细胞和豚鼠红细胞的差异吸附。

Differential adsorption of polyoma virions and capsids to mouse kidney cells and guinea pig erythrocytes.

作者信息

Bolen J B, Consigli R A

出版信息

J Virol. 1979 Nov;32(2):679-83. doi: 10.1128/JVI.32.2.679-683.1979.

Abstract

Adsorption of 125I-labeled polyoma virions and capsids to the surface of mouse kidney cells (MKC) and guinea pig erythrocytes was examined. Purified polyoma capsids lack the ability to compete with polyoma virions for specific binding sites on the surface of MKC. These same capsids were, however, able to block virion adsorption to guinea pig erythrocytes. UV-inactivated virions blocked cellular receptors on MKC and thus inhibited infectious virions from infecting the cells. Capsids were unable to inhibit virion infection of MKC. Adsorption of polyoma virions to MKC and infection of these cells were found to be independent of the ability of the virions to agglutinate guinea pig erythrocytes.

摘要

研究了¹²⁵I标记的多瘤病毒粒子和衣壳对小鼠肾细胞(MKC)及豚鼠红细胞表面的吸附作用。纯化的多瘤病毒衣壳缺乏与多瘤病毒粒子竞争MKC表面特异性结合位点的能力。然而,这些相同的衣壳能够阻断病毒粒子对豚鼠红细胞的吸附。紫外线灭活的病毒粒子可阻断MKC上的细胞受体,从而抑制感染性病毒粒子感染细胞。衣壳无法抑制多瘤病毒粒子对MKC的感染。发现多瘤病毒粒子对MKC的吸附及其对这些细胞的感染与病毒粒子凝集豚鼠红细胞的能力无关。

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