Yuen L K, Consigli R A
J Virol. 1983 Sep;47(3):620-5. doi: 10.1128/JVI.47.3.620-625.1983.
Polyomavirus was purified from infected mouse cell lysates under mild physiological conditions. When analyzed in a sucrose gradient, a major virus peak (240S) was identified. This sucrose-isolated virus could be divided into two populations based on its stability to CsCl gradient centrifugation. Members of the unstable population were shown to eject their DNA cores when subjected to CsCl gradient centrifugation, forming empty capsids, whereas the stable population was unaffected by the same CsCl treatment. Formaldehyde fixation of the 240S virus particles stabilized the virions and prevented ejection of DNA and generation of empty capsids. When formaldehyde-fixed 240S virus was examined with the electron microscope, only full virions were observed. These results indicate that polyoma capsids are not preformed in vivo, but instead are generated when infected cell lysates are subjected to harsh CsCl purification procedures.
多瘤病毒在温和的生理条件下从感染的小鼠细胞裂解物中纯化出来。在蔗糖梯度中进行分析时，鉴定出一个主要的病毒峰（240S）。这种通过蔗糖分离的病毒根据其对氯化铯梯度离心的稳定性可分为两个群体。不稳定群体的成员在进行氯化铯梯度离心时会排出其DNA核心，形成空衣壳，而稳定群体不受相同氯化铯处理的影响。对240S病毒颗粒进行甲醛固定可使病毒粒子稳定，并防止DNA排出和空衣壳的产生。当用电子显微镜检查甲醛固定的240S病毒时，只观察到完整的病毒粒子。这些结果表明，多瘤病毒衣壳并非在体内预先形成，而是在感染细胞裂解物经过苛刻的氯化铯纯化程序时产生的。
