适度给予乙醇对链脲佐菌素诱导的糖尿病Wistar大鼠生化指标的影响。

Effect of moderate ethanol administration on biochemical indices in streptozotocin-diabetic Wistar rats.

作者信息

Adaramoye O A, Oloyede G K

机构信息

Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, Faculty of Basic Medical Sciences, University of Ibadan, Nigeria.

出版信息

West Indian Med J. 2012 Jan;61(1):3-9. doi: 10.7727/wimj.2011.111.

DOI:10.7727/wimj.2011.111

PMID:22808559

Abstract

OBJECTIVE

This study was designed to evaluate the effect of moderate ethanol administration on the biochemical indices in streptozotocin (STZ)-diabetic rats.

METHODS

Twenty-four male Wistar rats were divided into four groups of six animals each. Groups one and two contained non-diabetic normal rats and normal rats treated with ethanol, respectively. Group three was untreated STZ-diabetic rats and group four was made up of ethanol-treated STZ-diabetic rats. Diabetes was induced by a single intraperitoneal injection of STZ (35 mg/kg), while ethanol (100%v/v) was given at a dose 2 g/kg thrice per week for three weeks. After the last dose of ethanol and an overnight fasting, rats were sacrificed by cervical dislocation. Blood was collected by syringe from the heart into plain centrifuge tubes.

RESULTS

Moderate ethanol administration to STZ-diabetic rats caused a significant (p < 0.05) increase in relative weight of liver relative to normal. Ethanol intake in STZ-diabetic rats produced an insignificant (p > 0.05) effect on the levels of fasting blood glucose (FBG) and HbA1c relative to the untreated-diabetic group. Moderately, ethanol administration to STZ-diabetic rats produced a marked and significant (p < 0.05) increase in the levels of serum total cholesterol, triglycerides, low-density lipoprotein (LDL)-cholesterol and the activities of alanine aminotransferase relative to untreated diabetic rats. Ethanol-treated diabetic rats had significantly (p < 0.05) lower high-density lipoprotein (HDL)-cholesterol levels, while the activities of lactate dehydrogenase and alpha-amylase were insignificantly (p > 0.05) affected. There were no significant (p > 0.05) differences in all the biochemical indices in normal rats relative to ethanol-treated normal rats.

CONCLUSIONS

Moderate ethanol administration did not affect FBG and HbA1c, but altered the lipid profile of STZ-diabetic rats. Moderate ethanol intake may further increase the risk of complications in diabetes.

摘要

目的

本研究旨在评估适量给予乙醇对链脲佐菌素（STZ）诱导的糖尿病大鼠生化指标的影响。

方法

将24只雄性Wistar大鼠分为四组，每组6只。第一组和第二组分别为非糖尿病正常大鼠和用乙醇处理的正常大鼠。第三组为未处理的STZ糖尿病大鼠，第四组为由用乙醇处理的STZ糖尿病大鼠组成。通过单次腹腔注射STZ（35mg/kg）诱导糖尿病，而乙醇（100%v/v）以2g/kg的剂量每周三次给药，共给药三周。在最后一剂乙醇给药后并过夜禁食后，通过颈椎脱臼处死大鼠。用注射器从心脏采血至普通离心管中。

结果

适量给予STZ糖尿病大鼠乙醇导致肝脏相对重量相对于正常大鼠显著增加（p<0.05）。相对于未处理的糖尿病组，STZ糖尿病大鼠摄入乙醇对空腹血糖（FBG）和糖化血红蛋白（HbA1c）水平产生的影响不显著（p>0.05）。适量给予STZ糖尿病大鼠乙醇相对于未处理的糖尿病大鼠使血清总胆固醇、甘油三酯、低密度脂蛋白（LDL）胆固醇水平以及丙氨酸转氨酶活性显著增加（p<0.05）。用乙醇处理的糖尿病大鼠的高密度脂蛋白（HDL）胆固醇水平显著降低（p<0.05），而乳酸脱氢酶和α淀粉酶的活性受到的影响不显著（p>0.05）。相对于用乙醇处理的正常大鼠，正常大鼠的所有生化指标均无显著差异（p>0.05）。