Ozyilmaz G, Ozyilmaz A T, Can F
University of Mustafa Kemal, Faculty of Arts and Sciences, Department of Chemistry, 31040 Hatay, Turkey.
Prikl Biokhim Mikrobiol. 2011 Mar-Apr;47(2):217-25.
Amperometric glucose biosensors have been developed based on entrapment on platinum (Pt) electrode using cyclic voltammetry technique in glucose oxidase (GOD) and pyrrole containing p-toluenesulfonic acid (pTSA) or sodium p-toluenesulfonate (NapTS) as supporting electrolyte solutions. Both of electrolyte solutions were suitable media for the formation and deposition of polypyrrole-GOD (PPy-GOD) layers on Pt substrate. Pt/PPy-GOD electrodes brought about in different morphological properties as well as different electrochemical and biochemical response. The highest responses obtained in pTSA and NapTS electrolytes were observed at pH of 4.5 and 7.0 for Pt/PPy-GOD electrodes, respectively. While linearity was observed between 0.0-1.0 mM glucose substrate for both electrodes, I(max) value of Pt/PPy-GOD(NapTS) electrode was approximately twice as high as that of Pt/PPy-GOD(pTSA) electrode as 25.4 and 14.2 microA, respectively. Five commercial drinks were tested with enzyme electrodes and compared with results obtained spectrophotometrically using glucose kit. Results revealed that Pt/PPy-GOD(NapTS) electrode exhibited better biosensor response.
基于循环伏安法技术，以葡萄糖氧化酶（GOD）和含有对甲苯磺酸（pTSA）或对甲苯磺酸钠（NapTS）的吡咯作为支持电解质溶液，将其包埋在铂（Pt）电极上，从而开发出了安培型葡萄糖生物传感器。这两种电解质溶液都是在Pt基底上形成和沉积聚吡咯 - GOD（PPy - GOD）层的合适介质。Pt/PPy - GOD电极具有不同的形态特性以及不同的电化学和生化响应。对于Pt/PPy - GOD电极，在pTSA和NapTS电解质中分别在pH为4.5和7.0时观察到最高响应。虽然两个电极在0.0 - 1.0 mM葡萄糖底物之间都观察到线性关系，但Pt/PPy - GOD（NapTS）电极的I(max)值分别为25.4和14.2微安，约为Pt/PPy - GOD（pTSA）电极的两倍。用酶电极对五种商业饮料进行了测试，并与使用葡萄糖试剂盒分光光度法获得的结果进行了比较。结果表明，Pt/PPy - GOD（NapTS）电极表现出更好的生物传感器响应。
