Experimental Biology Posgrade, Department of Biology of Reproduction, D.C.B.S., Universidad Autónoma Metropolitana Iztapalapa, México, DF, México.
Am J Chin Med. 2012;40(4):753-68. doi: 10.1142/S0192415X12500565.
It has been demonstrated that the decoction of the aerial parts of Tagetes lucida Cav. produces an antidepressant effect during the forced swimming test (FST) in rats. The aim of this study was to evaluate the effect of different organic extracts and one aqueous extract of the aerial parts of T. lucida on the FST. In addition, the possible involvement of the serotonergic system in the antidepressant-like effect of T. lucida in the FST was evaluated, as was its potential toxicological effect. The different extracts of T. lucida (methanol, hexane, dichloromethane and aqueous, 10 and 50 mg/kg), as well as fluoxetine (FLX, 5 mg/kg), were administered per os (p.o.) to rats for 14 days. All animals were subjected to the FST. Only the aqueous extract of T. lucida at a dose of 50 mg/kg significantly reduced immobility behavior and increased swimming in the FST, similar to FLX. Later, the aqueous extract of T. lucida (50mg/kg) was administered for 1, 7 and 14 days. An antidepressant effect was observed after 7 days of treatment. To evaluate the participation of the serotoninergic system, the animals were pretreated with PCPA, an inhibitor of serotonin synthesis (100 mg/kg/day for 4 consecutive days). The animals were treated with the aqueous extract of T. lucida (50 mg/kg) and FLX (5 mg/kg) 24 h after the final injection and were then subjected to the FST. Pretreatment with PCPA inhibited the antidepressant effect of both T. lucida and FLX. Finally, T. lucida was administered p.o. and intraperitoneal route to evaluate its acute toxicological effect. The aqueous extract of T. lucida, administered p.o., did not produce lethality or any significant changes in behavior. In conclusion, the aqueous extract of T. lucida manifested an antidepressant-like effect in the FST mediated by the serotonergic system, with no adverse effects when administered p.o.
已证实，Tagetes lucida Cav. 的地上部分的煎剂在大鼠强迫游泳试验（FST）中产生抗抑郁作用。本研究的目的是评估 T. lucida 的地上部分的不同有机提取物和一种水提取物对 FST 的影响。此外，还评估了 5-羟色胺能系统在 T. lucida 的 FST 中抗抑郁样作用的可能参与，以及其潜在的毒理学作用。T. lucida 的不同提取物（甲醇、己烷、二氯甲烷和水，10 和 50mg/kg）以及氟西汀（FLX，5mg/kg）以口服（p.o.）方式给药，连续 14 天给药。所有动物均进行 FST。只有 T. lucida 的水提取物在 50mg/kg 的剂量下可显著减少 FST 中的不动行为并增加游泳行为，与 FLX 相似。之后，T. lucida 的水提取物（50mg/kg）连续给药 1、7 和 14 天。在治疗 7 天后观察到抗抑郁作用。为了评估 5-羟色胺能系统的参与，动物预先用 PCPA 处理，PCPA 是一种 5-羟色胺合成抑制剂（连续 4 天每天 100mg/kg）。动物在最后一次注射后 24 小时用 T. lucida 的水提取物（50mg/kg）和 FLX（5mg/kg）处理，然后进行 FST。PCPA 预处理抑制了 T. lucida 和 FLX 的抗抑郁作用。最后，T. lucida 经口服和腹腔途径给药，以评估其急性毒理学作用。T. lucida 的水提取物经口服给药，没有产生致死性或任何行为的显著变化。总之，T. lucida 的水提取物在 FST 中表现出抗抑郁样作用，通过 5-羟色胺能系统介导，经口服给药没有不良反应。
