Machado Daniele G, Bettio Luis E B, Cunha Mauricio P, Capra Juliano C, Dalmarco Juliana B, Pizzolatti Moacir G, Rodrigues Ana Lúcia S
Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Brazil.
Prog Neuropsychopharmacol Biol Psychiatry. 2009 Jun 15;33(4):642-50. doi: 10.1016/j.pnpbp.2009.03.004. Epub 2009 Mar 13.
Rosemary, Rosmarinus officinalis L. (Labiatae) has several therapeutic applications in folk medicine in curing or managing a wide range of diseases, including depression. In this study, the effect of the hydroalcoholic extract of the stems and leaves of this plant was investigated in two behavioral models, the forced swimming test (FST) and tail suspension test (TST) in mice. The extract of R. officinalis produced an antidepressant-like effect, since the acute treatment of mice with the extract by p.o. route significantly reduced the immobility time in the FST (100 mg/kg) and TST (10-100 mg/kg), as compared to a control group, without accompanying changes in ambulation in the open-field test. Moreover, the repeated administration (14 days) of the hydroalcoholic extract of R. officinalis by p.o. route also produced an antidepressant-like effect in the TST (100-300 mg/kg). The pretreatment of mice with p-chlorophenylalanine (PCPA, 100 mg/kg, i.p., an inhibitor of serotonin synthesis, for 4 consecutive days), NAN-190 (0.5 mg/kg, i.p., a 5-HT(1A) receptor antagonist), ketanserin (5 mg/kg, i.p., a 5-HT(2A) receptor antagonist), 1-(m-chlorophenyl) biguanide (mCPBG, 10 mg/kg, i.p., a 5-HT(3) receptor agonist), prazosin (1 mg/kg, i.p., an alpha(1-)adrenoceptor antagonist), SCH23390 (0.05 mg/kg, s.c., a dopamine D(1) receptor antagonist) or sulpiride (50 mg/kg, i.p., a dopamine D(2) receptor antagonist), but not yohimbine (1 mg/kg, i.p., an alpha(2-)adrenoceptor antagonist) was able to reverse the anti-immobility effect of the extract (10 mg/kg, p.o.) in the TST. The combination of MDL72222, (0.1 mg/kg, i.p., a 5-HT(3) receptor antagonist) with a sub-effective dose of the extract of R. officinalis (1 mg/kg, p.o.) produced an anti-immobility effect in the TST. The results suggest that the antidepressant action of the extract of R. officinalis is mediated by an interaction with the monoaminergic system and that this plant should be further investigated as an alternative therapeutic approach for the treatment of depression.
迷迭香，即迷迭香叶唇形科植物（Rosmarinus officinalis L.），在民间医学中有多种治疗用途，可用于治疗或管理多种疾病，包括抑郁症。在本研究中，通过强迫游泳试验（FST）和小鼠尾悬挂试验（TST）这两种行为学模型，研究了该植物茎叶的水醇提取物的作用。迷迭香提取物产生了类似抗抑郁的效果，因为经口途径用该提取物急性处理小鼠后，与对照组相比，在FST（100 mg/kg）和TST（10 - 100 mg/kg）中显著减少了不动时间，且在旷场试验中行走情况没有伴随变化。此外，经口途径重复给予迷迭香水醇提取物（14天）在TST（100 - 300 mg/kg）中也产生了类似抗抑郁的效果。用对氯苯丙氨酸（PCPA，100 mg/kg，腹腔注射，一种血清素合成抑制剂，连续4天）、NAN - 190（0.5 mg/kg，腹腔注射，一种5 - HT(1A)受体拮抗剂）、酮色林（5 mg/kg，腹腔注射，一种5 - HT(2A)受体拮抗剂）、1 - （间氯苯基）双胍（mCPBG，10 mg/kg，腹腔注射，一种5 - HT(3)受体激动剂）、哌唑嗪（1 mg/kg，腹腔注射，一种α(1-)肾上腺素能受体拮抗剂）、SCH23390（0.05 mg/kg，皮下注射，一种多巴胺D(1)受体拮抗剂）或舒必利（50 mg/kg，腹腔注射，一种多巴胺D(2)受体拮抗剂）预处理小鼠，但用育亨宾（1 mg/kg，腹腔注射，一种α(2-)肾上腺素能受体拮抗剂）预处理小鼠则不能逆转提取物（10 mg/kg，经口）在TST中的抗不动效果。MDL72222（0.1 mg/kg，腹腔注射，一种5 - HT(3)受体拮抗剂）与亚有效剂量的迷迭香提取物（1 mg/kg，经口）联合使用在TST中产生了抗不动效果。结果表明，迷迭香提取物的抗抑郁作用是通过与单胺能系统相互作用介导的，并且该植物作为抑郁症治疗的替代治疗方法应进一步研究。
