Center for Regenerative Therapeutics and Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA.
Annu Rev Biomed Eng. 2012;14:325-49. doi: 10.1146/annurev-bioeng-071811-150041.
Stem cells hold significant promise for regeneration of tissue defects and disease-modifying therapies. Although numerous promising stem cell approaches are advancing in clinical trials, intraoperative stem cell therapies offer more immediate hope by integrating an autologous cell source with a well-established surgical intervention in a single procedure. Herein, the major developments in intraoperative stem cell approaches, from in vivo models to clinical studies, are reviewed, and the potential regenerative mechanisms and the roles of different cell populations in the regeneration process are discussed. Although intraoperative stem cell therapies have been shown to be safe and effective for several indications, there are still critical challenges to be tackled prior to adoption into the standard surgical armamentarium.
干细胞在组织缺陷的再生和疾病修饰疗法方面具有重要的应用前景。虽然有许多有前途的干细胞方法正在临床试验中推进，但术中干细胞疗法通过将自体细胞来源与单一手术中的成熟手术干预相结合，提供了更直接的希望。本文综述了术中干细胞方法从体内模型到临床研究的主要进展，并讨论了潜在的再生机制和不同细胞群体在再生过程中的作用。尽管术中干细胞疗法已被证明在多种适应症中是安全有效的，但在将其纳入标准手术工具之前，仍有一些关键的挑战需要解决。
