Hicok Kevin C, Hedrick Marc H
Regenerative Cell Therapeutics Research, Cytori Therapeutics, Inc., San Diego, CA, USA.
Methods Mol Biol. 2011;702:87-105. doi: 10.1007/978-1-61737-960-4_8.
The popularity of nonhematopoietic, adult tissue-derived stem and progenitor cells for use as a cellular research tool, and ultimately as a clinical therapeutic, has increased exponentially over the past decade. Almost all adult-derived stem/progenitor cells (autologous and allogeneic), with one exception, require at least some ex vivo expansion or further manipulation prior to use to satisfy efficacy and safety requirements for preclinical or clinical use. The principal reason is the relatively low frequency of these therapeutically valuable cells within any given adult tissue, except for adipose tissue, which has been shown to have at least two log greater concentrations of these progenitor cells. Therefore, use of autologous adipose-derived cells as both a research tool and cell therapeutic is feasible and has been shown to be both safe and efficacious in preclinical and clinical models of injury and disease. The development and utilization of automated processes and instrumentation such as Cytori Therapeutics' Celution® System to reduce variability and increase quality of the recovered cells is requisite for clinical use and preferred by basic researchers. Here, use of an automated, closed processing platform for isolation and concentration of adipose-derived stem and regenerative cells is described, including a profile of the isolated cells immediately prior to use, and commonly used methods to quantify and qualitatively assess the recovered cells.
在过去十年中，非造血的、源自成人组织的干细胞和祖细胞作为一种细胞研究工具，并最终作为一种临床治疗手段，其受欢迎程度呈指数级增长。几乎所有源自成人的干细胞/祖细胞（自体和异体），有一个例外，在使用前都需要至少进行一些体外扩增或进一步处理，以满足临床前或临床使用的疗效和安全要求。主要原因是在任何给定的成人组织中，除了脂肪组织外，这些具有治疗价值的细胞频率相对较低，而脂肪组织已被证明含有至少高两个对数浓度的这些祖细胞。因此，使用自体脂肪来源的细胞作为研究工具和细胞治疗手段是可行的，并且在损伤和疾病的临床前和临床模型中已被证明是安全有效的。开发和利用自动化流程和仪器，如Cytori Therapeutics公司的Celution®系统，以减少变异性并提高回收细胞的质量，这是临床使用所必需的，也是基础研究人员所青睐的。在此，描述了使用自动化的、封闭的处理平台来分离和浓缩脂肪来源的干细胞和再生细胞，包括使用前立即对分离细胞的概况，以及常用的定量和定性评估回收细胞的方法。
