顺势疗法制剂由山金车、金盏花、贯叶连翘和紫草组成，可加速 NIH 3T3 成纤维细胞体外划痕伤口闭合。
A homeopathic remedy from arnica, marigold, St. John's wort and comfrey accelerates in vitro wound scratch closure of NIH 3T3 fibroblasts.
机构信息
Institute for Complementary Medicine, University Hospital Zurich, Raemistrasse 100, Zurich 8091, Switzerland.
出版信息
BMC Complement Altern Med. 2012 Jul 18;12:100. doi: 10.1186/1472-6882-12-100.
BACKGROUND
Drugs of plant origin such as Arnica montana, Calendula officinalis or Hypericum perforatum have been frequently used to promote wound healing. While their effect on wound healing using preparations at pharmacological concentrations was supported by several in vitro and clinical studies, investigations of herbal homeopathic remedies on wound healing process are rare. The objective of this study was to investigate the effect of a commercial low potency homeopathic remedy Similasan® Arnica plus Spray on wound closure in a controlled, blind trial in vitro.
METHODS
We investigated the effect of an ethanolic preparation composed of equal parts of Arnica montana 4x, Calendula officinalis 4x, Hypericum perforatum 4x and Symphytum officinale 6x (0712-2), its succussed hydroalcoholic solvent (0712-1) and unsuccussed solvent (0712-3) on NIH 3T3 fibroblasts. Cell viability was determined by WST-1 assay, cell growth using BrdU uptake, cell migration by chemotaxis assay and wound closure by CytoSelect ™Wound Healing Assay Kit which generated a defined "wound field". All assays were performed in three independent controlled experiments.
RESULTS
None of the three substances affected cell viability and none showed a stimulating effect on cell proliferation. Preparation (0712-2) exerted a stimulating effect on fibroblast migration (31.9%) vs 14.7% with succussed solvent (0712-1) at 1:100 dilutions (p < 0.001). Unsuccussed solvent (0712-3) had no influence on cell migration (6.3%; p > 0.05). Preparation (0712-2) at a dilution of 1:100 promoted in vitro wound closure by 59.5% and differed significantly (p < 0.001) from succussed solvent (0712-1), which caused 22.1% wound closure.
CONCLUSION
Results of this study showed that the low potency homeopathic remedy (0712-2) exerted in vitro wound closure potential in NIH 3T3 fibroblasts. This effect resulted from stimulation of fibroblasts motility rather than of their mitosis.
背景
植物来源的药物，如山金车、金盏花或贯叶连翘，已被广泛用于促进伤口愈合。虽然一些体外和临床研究支持这些药物在药理学浓度下对伤口愈合的作用，但对草药顺势疗法治疗伤口愈合过程的研究却很少。本研究的目的是在体外对照、盲法试验中研究一种商业低浓度顺势疗法 Similasan®山金车加喷雾剂对伤口闭合的影响。
方法
我们研究了一种由山金车 4x、金盏花 4x、贯叶连翘 4x 和筋骨草 6x 等份组成的乙醇制剂（0712-2）、其摇动的水醇溶剂（0712-1）和未摇动的溶剂（0712-3）对 NIH 3T3 成纤维细胞的影响。通过 WST-1 测定法测定细胞活力，通过 BrdU 摄取测定细胞生长，通过趋化性测定法测定细胞迁移，通过 CytoSelect ™伤口愈合测定试剂盒测定伤口闭合，该试剂盒生成一个定义的“伤口场”。所有测定均在三个独立的对照实验中进行。
结果
三种物质均不影响细胞活力，也不显示对细胞增殖有刺激作用。制剂（0712-2）在 1:100 稀释度下对成纤维细胞迁移有刺激作用（31.9%），而摇动溶剂（0712-1）为 14.7%（p <0.001）。未摇动的溶剂（0712-3）对细胞迁移无影响（6.3%；p>0.05）。在 1:100 的稀释度下，制剂（0712-2）促进体外伤口闭合 59.5%，与摇动溶剂（0712-1）的 22.1%伤口闭合差异显著（p<0.001）。
结论
本研究结果表明，低浓度顺势疗法（0712-2）在 NIH 3T3 成纤维细胞中具有体外伤口闭合潜力。这种作用是通过刺激成纤维细胞的运动而不是有丝分裂产生的。
Zotero 插件