Society for Cancer Research, Hiscia Institute, 4144 Arlesheim, Switzerland.
Institute for Complementary Medicine, University Hospital Zurich, 8091 Zurich, Switzerland.
Evid Based Complement Alternat Med. 2013;2013:718105. doi: 10.1155/2013/718105. Epub 2013 Nov 26.
Viscum album L. lipophilic extract (VALE) contains pharmacologically active pentacyclic triterpenes that are known to exhibit immunomodulatory, antitumor, and wound healing activity. Preliminary clinical observations indicate that VALE was able to influence cutaneous wound healing in vivo. The objective of this study was to investigate wound closure related properties of VALE in vitro. As measured in a wound healing assay, VALE and its predominant triterpene oleanolic acid (OA) significantly and dose dependently promoted the migration of NIH/3T3 fibroblasts in vitro, thereby leading to an enhanced wound closure. Compared to the negative control, maximal stimulation by 26.1% and 26.2%, respectively, was attained with 10 μg/mL VALE and 1 μg/mL OA. Stimulation of proliferation in NIH/3T3 fibroblasts by VALE and OA could be excluded. At higher concentrations both substances affected proliferation and viability of NIH/3T3 fibroblasts and HaCat keratinocytes. In the toxic range of concentrations of VALE and OA, migration of NIH/3T3 fibroblasts was suppressed. The extent of the stimulatory effect on cell migration of VALE quite closely corresponded to the effect expected by the concentrations of OA contained in the crude extract VALE. These data support the casual observation that Viscum album L. lipophilic extract might modulate wound healing related processes in vivo.
槲寄生亲脂提取物（VALE）含有具有药理活性的五环三萜类化合物，已知其具有免疫调节、抗肿瘤和伤口愈合活性。初步临床观察表明，VALE 能够影响体内皮肤伤口愈合。本研究的目的是研究 VALE 在体外与伤口闭合相关的特性。如在伤口愈合测定中所测量的，VALE 及其主要三萜齐墩果酸（OA）显著且剂量依赖性地促进 NIH/3T3 成纤维细胞的体外迁移，从而增强伤口闭合。与阴性对照相比，10μg/ml 的 VALE 和 1μg/ml 的 OA 分别达到最大刺激 26.1%和 26.2%。VALE 和 OA 对 NIH/3T3 成纤维细胞的增殖刺激可以排除。在较高浓度下，这两种物质均影响 NIH/3T3 成纤维细胞和 HaCat 角质形成细胞的增殖和活力。在 VALE 和 OA 的毒性浓度范围内，NIH/3T3 成纤维细胞的迁移受到抑制。VALE 对细胞迁移的刺激作用的程度与粗提物 VALE 中所含 OA 浓度的预期作用非常吻合。这些数据支持了一个偶然的观察结果，即槲寄生亲脂提取物可能调节体内与伤口愈合相关的过程。
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