Department of Biochemistry, Laboratory of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, PO Box 67, Dschang, Cameroon.
BMC Complement Altern Med. 2012 Jul 18;12:99. doi: 10.1186/1472-6882-12-99.
Albizia adianthifolia is used traditionally in Cameroon to treat several ailments, including infectious and associated diseases. This work was therefore designed to investigate the antioxidant and antimicrobial activities of ethyl acetate extract, fractions and compounds isolated from the stem bark of this plant.
The plant extract was prepared by maceration in ethyl acetate. Its fractionation was done by column chromatography and the structures of isolated compounds were elucidated using spectroscopic data in conjunction with literature data. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and trolox equivalent antioxidant capacity (TEAC) assays were used to detect the antioxidant activity. Broth micro-dilution method was used for antimicrobial test. Total phenol content was determined spectrophotometrically in the extracts by using Folin-Ciocalteu method.
The fractionation of the extract afforded two known compounds: lupeol (1) and aurantiamide acetate (2) together with two mixtures of fatty acids: oleic acid and n-hexadecanoic acid (B₁); n-hexadecanoic acid, octadecanoic acid and docosanoic acid (B₂). Aurantiamide acetate was the most active compound. The total phenol concentration expressed as gallic acid equivalents (GAE) was found to vary from 1.50 to 13.49 μg/ml in the extracts. The antioxidant activities were well correlated with the total phenol content (R² = 0.946 for the TEAC method and R² = 0.980 for the DPPH free-radical scavenging assay).
Our results clearly reveal that the ethyl acetate extract from the stem bark of A. adianthifolia possesses antioxidant and antimicrobial principles. The antioxidant activity of this extract as well as that of compound 2 are being reported herein for the first time. These results provide promising baseline information for the potential use of this plant as well as compound 2 in the treatment of oxidative damage and infections associated with the studied microorganisms.
在喀麦隆，阿比亚迪亚尼菲拉被传统用于治疗多种疾病，包括传染病和相关疾病。因此，这项工作旨在研究该植物茎皮的乙酸乙酯提取物、馏分和化合物的抗氧化和抗菌活性。
通过在乙酸乙酯中浸提制备植物提取物。通过柱色谱对其进行馏分分离，通过光谱数据与文献数据相结合来阐明分离化合物的结构。使用 1,1-二苯基-2-苦基肼（DPPH）和 Trolox 等效抗氧化能力（TEAC）测定法来检测抗氧化活性。使用肉汤微量稀释法进行抗菌试验。通过福林-肖卡泰尔法在提取物中分光光度法测定总酚含量。
提取物的馏分得到了两种已知化合物：羽扇豆醇（1）和aurantiamide 乙酸盐（2）以及两种脂肪酸混合物：油酸和正十六烷酸（B₁）；正十六烷酸、十八烷酸和二十二烷酸（B₂）。 Aurantiamide 乙酸盐是最活跃的化合物。总酚浓度以没食子酸当量（GAE）表示，在提取物中变化范围为 1.50 至 13.49μg/ml。抗氧化活性与总酚含量密切相关（TEAC 法的 R²为 0.946，DPPH 自由基清除测定法的 R²为 0.980）。
我们的结果清楚地表明，阿比亚迪亚尼菲拉茎皮的乙酸乙酯提取物具有抗氧化和抗菌成分。本文首次报道了该提取物和化合物 2 的抗氧化活性。这些结果为该植物以及化合物 2 作为治疗研究微生物相关的氧化损伤和感染的潜在用途提供了有希望的基线信息。
