Okusa P N, Penge O, Devleeschouwer M, Duez P
Laboratoire de Pharmacognosie, de Bromatologie et de Nutrition Humaine, Institut de Pharmacie, Université Libre de Bruxelles, CP 205/09, Boulevard du Triomphe, 1050 Brussels, Belgium.
J Ethnopharmacol. 2007 Jul 25;112(3):476-81. doi: 10.1016/j.jep.2007.04.003. Epub 2007 Apr 24.
Cordia gilletii De Wild (Boraginaceae) root bark is traditionally used in Democratic Republic of Congo (DRC) for the treatment of various disorders, including malaria, diarrhea, wounds and skin diseases; part of these activities may rely on antimicrobial and antioxidant properties. Successive extracts of root barks powder with n-hexane, dichloromethane, ethyl acetate, methanol and water were tested for antimicrobial activity, both direct and indirect (antibiotic resistance reversal), against 10 strains of bacteria and 1 strain of fungi by broth microdilution and agar diffusion methods. The eventual synergy between plant extracts and antibiotics was investigated by the determination of the fractional inhibitory concentration index (FIC index). The methanol extract showed direct antimicrobial activity against all tested microorganisms with minimum inhibitory concentrations (MIC) ranging between 125 and 1000 microg/ml, whereas the ethyl acetate and the dichloromethane extracts showed activity on four and three strains, respectively. 200 microg/ml of n-hexane and dichloromethane extracts decreased the MICs of penicillin and streptomycin 4-64-fold for methicillin-resistant Staphylococcus aureus. A synergistic effect was found between the methanol extract and tetracycline, whereas additive effects were observed for the other combinations tested. The methanol and dichloromethane extracts showed the greater antioxidant activity by scavenging the free radical DPPH with IC(50) values of 3.2 and 8.1 microg/ml, respectively. These results support the use of the plant in the treatment of infectious diseases and wounds; they warrant further studies as to the nature of active compounds.
吉利特破布木（紫草科）的根皮在刚果民主共和国传统上用于治疗各种疾病，包括疟疾、腹泻、伤口和皮肤病；这些功效部分可能依赖于抗菌和抗氧化特性。采用肉汤微量稀释法和琼脂扩散法，对用正己烷、二氯甲烷、乙酸乙酯、甲醇和水依次提取的根皮粉末提取物针对10种细菌菌株和1种真菌菌株进行了直接和间接（抗生素抗性逆转）抗菌活性测试。通过测定分数抑制浓度指数（FIC指数）研究了植物提取物与抗生素之间最终的协同作用。甲醇提取物对所有测试微生物均显示出直接抗菌活性，最低抑菌浓度（MIC）在125至1000微克/毫升之间，而乙酸乙酯提取物和二氯甲烷提取物分别对4种和3种菌株有活性。200微克/毫升的正己烷和二氯甲烷提取物可使耐甲氧西林金黄色葡萄球菌对青霉素和链霉素的MIC降低4至64倍。发现甲醇提取物与四环素之间有协同作用，而对其他测试组合观察到的是相加作用。甲醇提取物和二氯甲烷提取物通过清除自由基DPPH表现出更强的抗氧化活性，其IC（50）值分别为3.2和8.1微克/毫升。这些结果支持该植物在治疗传染病和伤口方面的应用；它们需要对活性化合物的性质进行进一步研究。
