School of Plant, Environmental, and Soil Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.
Biochem Biophys Res Commun. 2012 Aug 10;424(4):747-52. doi: 10.1016/j.bbrc.2012.07.023. Epub 2012 Jul 15.
Salinity and drought are the most important environmental constraints limiting crop growth and productivity. Here, we have characterized a gene 'SaβNAC' encoding the β subunit of nascent polypeptide associated complex from a halophyte Spartina alterniflora and investigated its role toward abiotic stress regulation. Expression of SaβNAC was differentially regulated by abiotic stresses, including salinity, drought, cold, and ABA in leaves and roots of S. alterniflora. Constitutive over-expression of SaβNAC in Arabidopsis exhibited normal growth under non-stress conditions but enhanced tolerance to salt and drought stresses. Transgenic SaβNAC Arabidopsis retained more chlorophyll, proline, and showed improved ionic homeostasis with less damage under stress conditions compared to WT plants. This is a first report to demonstrate the involvement of βNAC in imparting abiotic stress tolerance which might be due to protection of the newly synthesized polypeptides involved in various stress tolerance mechanisms from abiotic stress induced damage and inhibition of cell death in plant.
盐度和干旱是限制作物生长和生产力的最重要的环境限制因素。在这里，我们从盐生植物互花米草中鉴定了一个编码新生多肽相关复合物β亚基的基因 'SaβNAC'，并研究了它在非生物胁迫调节中的作用。SaβNAC 的表达受非生物胁迫的差异调节，包括盐度、干旱、寒冷和 ABA 在互花米草的叶片和根中。SaβNAC 在拟南芥中的组成型过表达在非胁迫条件下表现出正常的生长，但增强了对盐和干旱胁迫的耐受性。与 WT 植物相比，转基因 SaβNAC 拟南芥在胁迫条件下保留了更多的叶绿素、脯氨酸，表现出更好的离子稳态，损伤较小。这是第一个证明 βNAC 参与赋予非生物胁迫耐受性的报告，这可能是由于保护新合成的多肽免受非生物胁迫诱导的损伤和抑制植物细胞死亡，这些多肽参与各种胁迫耐受机制。
