Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
Cold Spring Harb Perspect Biol. 2012 Oct 1;4(10):a011544. doi: 10.1101/cshperspect.a011544.
Translation initiation in eukaryotes is a highly regulated and complex stage of gene expression. It requires the action of at least 12 initiation factors, many of which are known to be the targets of regulatory pathways. Here we review our current understanding of the molecular mechanics of eukaryotic translation initiation, focusing on recent breakthroughs from in vitro and in vivo studies. We also identify important unanswered questions that will require new ideas and techniques to solve.
真核生物的翻译起始是一个高度调控和复杂的基因表达阶段。它需要至少 12 种起始因子的作用，其中许多因子是已知的调节途径的靶标。在这里，我们回顾了我们对真核翻译起始的分子机制的现有认识，重点介绍了来自体外和体内研究的最新突破。我们还确定了一些重要的未解决问题，这些问题需要新的思路和技术来解决。
