Serdar Berrin, Lee David, Dou Zihong
Department of Environmental and Occupational Health, Robert Stempel College of Public Health and Social Work, Florida International University, Aurora, Colorado, USA.
BMJ Open. 2012 Jul 19;2(4). doi: 10.1136/bmjopen-2012-001318. Print 2012.
The main goal of this pilot study was to assess the technical and logistic feasibility of a future study. The research hypothesis is that occupational exposures to polycyclic aromatic hydrocarbons (PAHs) are associated with increased risk of DNA damage among roofers who work with hot asphalt.
This is a cross-sectional pilot study.
The study included roofers from four different construction sites in Miami-Dade County, Florida.
19 roofers were recruited (six Hispanics and 13 African-Americans, all male), all of whom were eligible (no history of cancer and no history of chronic diseases of kidneys or liver). All participants provided pre-shift samples and 18 provided post-shift samples. Samples of one participant were excluded from the final analyses as they were considered unreliable.
Levels of urinary PAH metabolites increased during 6 h of work. Linear regression models of post-shift metabolites included their pre-shift levels, post-shift urinary creatinine levels (for models of 1-OHPyr and 9-OHPhe), and skin burn due to contact with hot asphalt (for models of 1-OHPyr and 1-OHNap). Pre-shift levels of urinary 8-OHdG were not associated with any of the variables considered. For post-shift levels of 8-OHdG, however, post-shift 1-OHPyr (95% CI 0.091 to 0.788) and use of protective gloves (95% CI -1.57 to -0.61) during work explained 86.8% of its variation. Overall, highest levels of urinary PAH metabolites and of 8-OHdG were observed among workers who reported having skin burn and who did not use gloves during work.
Urinary 1-OHPyr is a promising predictor of oxidative DNA damage among roofers. Work-related skin burn and use of protective gloves appear to influence PAH exposure and DNA damage levels in this group, suggesting the importance of dermal absorption.
本初步研究的主要目标是评估未来一项研究的技术和后勤可行性。研究假设是，职业性接触多环芳烃（PAHs）与从事热沥青作业的屋顶工DNA损伤风险增加有关。
这是一项横断面初步研究。
该研究纳入了佛罗里达州迈阿密-戴德县四个不同建筑工地的屋顶工。
招募了19名屋顶工（6名西班牙裔和13名非裔美国人，均为男性），他们均符合条件（无癌症病史，无肾脏或肝脏慢性疾病史）。所有参与者均提供了班前样本，18人提供了班后样本。一名参与者的样本因被认为不可靠而被排除在最终分析之外。
工作6小时期间尿中PAH代谢物水平升高。班后代谢物的线性回归模型包括班前水平、班后尿肌酐水平（用于1-OHPyr和9-OHPhe模型）以及因接触热沥青导致的皮肤灼伤（用于1-OHPyr和1-OHNap模型）。尿8-OHdG的班前水平与所考虑的任何变量均无关联。然而，对于班后8-OHdG水平，班后1-OHPyr（95%CI 0.091至0.788）以及工作期间使用防护手套（95%CI -1.57至-0.61）解释了其86.8%的变异。总体而言，在报告有皮肤灼伤且工作期间未使用手套的工人中，观察到尿中PAH代谢物和8-OHdG的水平最高。
尿1-OHPyr是屋顶工氧化DNA损伤的一个有前景的预测指标。与工作相关的皮肤灼伤和防护手套的使用似乎会影响该组中的PAH暴露和DNA损伤水平，表明皮肤吸收的重要性。
