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使用酶联免疫吸附测定法检测血浆孕酮以促进家兔饲养管理。

Use of an ELISA for plasma progesterone to facilitate rabbit husbandry.

作者信息

Morrell J M

机构信息

National Institute for Medical Research, The Ridgeway, Mill Hill, London.

出版信息

Vet Rec. 1990 Nov 24;127(21):521-4.

PMID:2281586
Abstract

There is a need for a simple and accurate method for detecting pregnancy in rabbits; the available methods are not ideal and may not provide the diagnosis at an appropriate time for the early remating of non-pregnant animals. This paper describes the use of an ELISA kit to measure progesterone concentrations in rabbit plasma; a qualitative assessment of the results appears to be sufficiently accurate for pregnancy diagnosis, as does the use of serum instead of plasma. The technique can be used to predict ovulation and to distinguish between pregnant and pseudopregnant animals.

摘要

需要一种简单而准确的方法来检测兔子是否怀孕;现有的方法并不理想,可能无法在合适的时间为未怀孕的动物进行早期再次配种提供诊断。本文描述了使用酶联免疫吸附测定(ELISA)试剂盒来测量兔血浆中的孕酮浓度;结果的定性评估对于妊娠诊断似乎足够准确,使用血清代替血浆时也是如此。该技术可用于预测排卵,并区分怀孕和假孕动物。

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