利用对 pH 敏感的比率型镧系探针实时细胞成像研究溶酶体 pH 变化。
Live cell imaging of lysosomal pH changes with pH responsive ratiometric lanthanide probes.
机构信息
Department of Chemistry, Durham University, South Road, Durham DH1 3LE, UK.
出版信息
Chem Commun (Camb). 2012 Sep 4;48(68):8520-2. doi: 10.1039/c2cc34267g. Epub 2012 Jul 23.
PMID:22820610
Abstract
Europium and terbium complexes of two structurally related ligands have been evaluated as optical probes to monitor changes in lysosomal pH; calibration using ionophores and fluorescent probes allows monitoring of the time dependence of lysosomal pH change, examining the green/red intensity ratio from internalised Tb-Eu complexes.
摘要
已评估两种结构相关配体的铕和铽配合物作为光学探针，以监测溶酶体 pH 值的变化；使用离子载体和荧光探针进行校准可监测溶酶体 pH 值变化的时间依赖性，从内化的 Tb-Eu 配合物检查其绿光/红光强度比。
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