Mandal T K
Biophysics Division, Saha Institute of Nuclear Physics, Calcutta, India.
Z Naturforsch C J Biosci. 1990 Jul-Aug;45(7-8):902-10. doi: 10.1515/znc-1990-7-824.
Polymyxin B produces dose dependent changes in the surface topography of pathogenic Vibrio cholerae cells. The susceptibilities of various vibrio strains to PB are also studied through analytical techniques. Statistical analysis shows significant differences among the four vibrios with regard to their sensitivities to PB, the classical strains being the most sensitive. Treating the classical strain with subinhibitory concentration of PB, we observed with both SEM and TEM that the normal smooth surface of the cell envelope develops some protruded structures (blebs and crenations). Further the TEM study of the ultrathin sections reveal that the rod like projections are formed by protrusions of the outer-membrane of the cell wall.
多粘菌素B会使致病性霍乱弧菌细胞的表面形貌产生剂量依赖性变化。还通过分析技术研究了各种弧菌菌株对多粘菌素B的敏感性。统计分析表明，这四种弧菌对多粘菌素B的敏感性存在显著差异，经典菌株最为敏感。用亚抑制浓度的多粘菌素B处理经典菌株，我们通过扫描电子显微镜和透射电子显微镜观察到，细胞膜正常的光滑表面出现了一些突出结构（泡状和锯齿状）。此外，对超薄切片的透射电子显微镜研究表明，杆状突起是由细胞壁外膜的突出形成的。
