Wan Z L, Liang D C
Institute of Biophysics, Academia Sinica, Beijing, PRC.
Sci China B. 1990 Jul;33(7):810-20.
We have determined the crystal structure of A1-(D-Trp) insulin and discovered that it belongs to the trigonal system with space group R3. The parameters of the unit cell are a = b = 78.6 A, c = 50.0 A. A set of data for half a sphere reciprocal space to a spacing of 2.2 A were collected. The model was adjusted and refined by using a step-by-step approach and a stereochemically-restrained least squares program, assisted by manual revision based on the difference Fourier maps, to a final R-factor of 0.218. The main and side chains of both A1-D-Trp residues in the asymmetric unit are well ordered. The packing of A1-(D-Trp) insulin in the unit cell, the conformational differences with other insulin structures and its structure and function relationship have also been discussed.
我们已经确定了A1-（D-色氨酸）胰岛素的晶体结构，并发现它属于三方晶系，空间群为R3。晶胞参数为a = b = 78.6 Å，c = 50.0 Å。收集了一组倒易空间半球到2.2 Å间距的数据。通过逐步方法和立体化学约束最小二乘法程序对模型进行调整和精修，并基于差值傅里叶图进行人工修正，最终R因子为0.218。不对称单元中两个A1-D-色氨酸残基的主链和侧链排列良好。还讨论了A1-（D-色氨酸）胰岛素在晶胞中的堆积、与其他胰岛素结构的构象差异及其结构与功能的关系。
