Clarke J R, Krishnan V, Bennett J, Mitchell D, Jeffries D J
Department of Medical Microbiology, St Mary's Hospital Medical School, London, UK.
AIDS. 1990 Nov;4(11):1133-6. doi: 10.1097/00002030-199011000-00012.
We have used the polymerase chain reaction to investigate HIV-1 infection of lung macrophages from 44 AIDS patients with pneumonia. Proviral HIV-1 DNA was detected in the lung macrophages of 47% (21 of 44) of patients tested; 32% (seven of 21) of the patients had Pneumocystis carinii pneumonia (PCP), and 56% (11 of 21) had pneumonia caused by other aetiological agents. Matched peripheral blood specimens were obtained from 22 patients in this study, and HIV-1-specific DNA was detected in 100% (22 out of 22) of non-adherent white cell preparations tested. HIV was detected in blood monocytes of 11 out of 22 patients (50%), and a virus signal was also found in the lung macrophages of eight of these patients. Our results indicate that PCP does not predispose lung macrophages to HIV-1, nor does HIV-1 infection of lung macrophages increase the probability of contracting PCP.
我们采用聚合酶链反应来研究44例患有肺炎的艾滋病患者肺巨噬细胞的HIV-1感染情况。在接受检测的患者中，47%（44例中的21例）的肺巨噬细胞中检测到了HIV-1前病毒DNA；这些患者中，32%（21例中的7例）患有卡氏肺孢子虫肺炎（PCP），56%（21例中的11例）患有由其他病原体引起的肺炎。本研究从22例患者中获取了配对的外周血标本，在检测的所有非贴壁白细胞制剂中，100%（22例中的22例）检测到了HIV-1特异性DNA。22例患者中有11例（50%）的血液单核细胞中检测到了HIV，其中8例患者的肺巨噬细胞中也发现了病毒信号。我们的结果表明，PCP不会使肺巨噬细胞更易感染HIV-1，肺巨噬细胞的HIV-1感染也不会增加患PCP的概率。
