利用聚合酶链反应检测人类外周血单个核细胞不同亚群中的HIV-1 DNA。快速通讯。
Detection of HIV-1 DNA in different subsets of human peripheral blood mononuclear cells using the polymerase chain reaction. Rapid communication.
作者信息
Hufert F T, von Laer D, Schramm C, Tárnok A, Schmitz H
机构信息
Virological Department, Bernhard-Nocht Institute of Tropical Medicine, Hamburg, Federal Republic of Germany.
出版信息
Arch Virol. 1989;106(3-4):341-5. doi: 10.1007/BF01313963.
The presence of HIV-1 proviral DNA was determined in CD4 cells, CD8 cells and macrophages/monocytes obtained from peripheral blood of 8 HIV-1 infected persons. Using the polymerase chain reaction (PCR) we were able to detect proviral DNA in the extracts of only 10(2)-10(3) CD4 (T4) cells. In contrast, 10(5) CD8 cells did not contain detectable amounts of proviral DNA. Surprisingly, in four of our eight patients studied no HIV-1 DNA was found in macrophages. In peripheral blood, every hundred T4 cell may be infected with HIV-1.
在8名HIV-1感染者外周血获取的CD4细胞、CD8细胞和巨噬细胞/单核细胞中检测HIV-1前病毒DNA的存在情况。使用聚合酶链反应（PCR），我们仅能在10²-10³个CD4（T4）细胞的提取物中检测到前病毒DNA。相比之下，10⁵个CD8细胞中未检测到可检测量的前病毒DNA。令人惊讶的是，在我们研究的8名患者中有4名患者的巨噬细胞中未发现HIV-1 DNA。在外周血中，每一百个T4细胞可能感染HIV-1。
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