Zheng Jie, Liu Duan, Zhao Su-qing, Su Juan, Yan Qiu-ping, Chen Lei, Xiao Yi, Zhang Chun-mei
Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China.
Zhong Yao Cai. 2012 Feb;35(2):304-6.
To investigate the technology of Aucubin in Eucommia ulmoides leaves extracted by enzymatic method and its antibacterial activity.
Aucubin in Eucommia ulmoides leaves was extracted by cellulase method. The extraction technology was optimized using the content of Aucubin as index. The antibacterial activity of Aucubin was determined.
The results showed that the optimum technology was as follows; The solid-liquid ratio was 1:12, extracted for 50 min by 0.4% enzyme at 50 degrees C in pH 6.0. The extraction rate of Aucubin was as high as 17.892 mg/g. The Aucubin extracted could obviously inhibit the growth of Escherichia coli and Staphylococcus aureus, the MIC of Aucubin against Staphylococcus aureus and Escherichia coli were 4.832 mg/mL and 9.664 mg/mL respectively. However, Aucubin presented weak inhibitory effect on Streptococcus pneumonia and MG-hemolytic streptococcus, the MIC of Aucubin against them were all 28.946 mg/mL.
The extraction technology obtained in this experiment is reasonable and feasible with high extraction rate, and the Aucubin has some antibacterial activity.
研究杜仲叶中桃叶珊瑚苷的酶法提取工艺及其抗菌活性。
采用纤维素酶法提取杜仲叶中的桃叶珊瑚苷。以桃叶珊瑚苷含量为指标对提取工艺进行优化。测定桃叶珊瑚苷的抗菌活性。
结果表明，最佳工艺如下：固液比为1:12，在pH 6.0、50℃条件下用0.4%的酶提取50 min。桃叶珊瑚苷的提取率高达17.892 mg/g。提取的桃叶珊瑚苷能明显抑制大肠杆菌和金黄色葡萄球菌的生长，桃叶珊瑚苷对金黄色葡萄球菌和大肠杆菌的最低抑菌浓度分别为4.832 mg/mL和9.664 mg/mL。然而，桃叶珊瑚苷对肺炎链球菌和溶血性链球菌的抑制作用较弱，其对它们的最低抑菌浓度均为28.946 mg/mL。
本实验得到的提取工艺合理可行，提取率高，且桃叶珊瑚苷具有一定的抗菌活性。
