Li Xiang-Zhou, Zhang Sheng
College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China; State Key Laboratory of Ecological Applied Technology in Forest Area of South China, Changsha, Hunan 410004, China.
College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China.
Evid Based Complement Alternat Med. 2016;2016:6362094. doi: 10.1155/2016/6362094. Epub 2016 Aug 28.
Eucommia ulmoides Oliv. (E. ulmoides Oliv.) and moso bamboo (Phyllostachys pubescens) leaves are used as folk medicines in central-western China to treat diabetes. To investigate the hypoglycemic activity of the effervescent granules prepared using E. ulmoides Oliv. and moso bamboo leaves (EBEG) in HepG2 cells, EBEG were prepared with 5% of each of polysaccharides and chlorogenic acids from moso bamboo and E. ulmoides Oliv. leaves, respectively. HepG2 cells cultured in a high-glucose medium were classified into different groups. The results displayed EBEG-treated cells showed better glucose utilization than the negative controls; thus, the hypoglycemic effect of EBEG was much greater than that of granules prepared using either component alone, thereby indicating that this effect was due to a synergistic action of the components. Further, glucose consumption levels in the cells treated with EBEG (156.35% at 200 μg/mL) and the positive controls (metformin, 162.29%; insulin, 161.52%) were similar. Thus, EBEG exhibited good potential for use as a natural antidiabetic agent. The hypoglycemic effect of EBEG could be due to the synergistic action of polysaccharides from the moso bamboo leaves and chlorogenic acids from E. ulmoides Oliv. leaves via the inhibition of alpha-glucosidase and glucose-6-phosphate displacement enzyme.
杜仲（Eucommia ulmoides Oliv.）和毛竹叶（Phyllostachys pubescens）在中国中西部地区被用作民间药物治疗糖尿病。为了研究用杜仲和毛竹叶制备的泡腾颗粒（EBEG）在HepG2细胞中的降血糖活性，分别用毛竹和杜仲叶中5%的多糖和绿原酸制备了EBEG。将在高糖培养基中培养的HepG2细胞分为不同组。结果显示，经EBEG处理的细胞比阴性对照组具有更好的葡萄糖利用率；因此，EBEG的降血糖作用远大于单独使用任何一种成分制备的颗粒，这表明这种作用是由于成分之间的协同作用。此外，用EBEG（200μg/mL时为156.35%）和阳性对照（二甲双胍，162.29%；胰岛素，161.52%）处理的细胞中的葡萄糖消耗水平相似。因此，EBEG作为天然抗糖尿病药物具有良好的应用潜力。EBEG的降血糖作用可能是由于毛竹叶多糖和杜仲叶绿原酸通过抑制α-葡萄糖苷酶和葡萄糖-6-磷酸移位酶的协同作用。
