免疫化学评价兔松果体 S 抗原。

Immunochemical evaluation of S-antigen of rabbit pineal gland.

机构信息

Department of Ophthalmology, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA.

出版信息

Ocul Immunol Inflamm. 1995;3(3):171-80. doi: 10.3109/09273949509069110.

Abstract

Purified S-antigen of photoreceptor cells induces experimental autoimmune uveoretinitis (EAU) and experimental autoimmune pinealitis (EAP) in laboratory animals. However, in rabbits, S-antigen induces only EAU without EAP. To evaluate this difference, the authors studied immunochemical reactivity of rabbit pineal gland with a panel of anti-S-antigen monoclonal antibodies (MAb). Rabbit pineal gland reacted with the MAbs by ELISA and immunoblot but not by immunohistochemistry. In contrast, rabbit retina like guinea pig retina, guinea pig pineal gland and bovine retina reacted with these MAbs by immunohistochemistry as well as by ELISA and immunoblot. Also, S-antigen purified from rabbit retina reacted as did bovine and guinea pig S-antigen. Therefore, S-antigen in situ in rabbit pineal gland is different from S-antigen of rabbit retina and different from S-antigen of pineal gland and retina of other species. Just as the MAbs did not react with S-antigen in rabbit pineal gland, it is possible that S-antigen activated lymphocytes may not recognize S-antigen in rabbit pineal gland and thereby not induce EAP.

摘要

感光细胞的纯化 S 抗原可在实验动物中诱导实验性自身免疫性葡萄膜炎（EAU）和实验性自身免疫性松果体炎（EAP）。然而，在兔子中，S 抗原仅诱导 EAU 而不诱导 EAP。为了评估这种差异，作者用一组抗 S 抗原单克隆抗体（MAb）研究了兔松果体的免疫化学反应性。兔松果体通过 ELISA 和免疫印迹与 MAb 发生反应，但通过免疫组织化学不发生反应。相比之下，兔视网膜与豚鼠视网膜、豚鼠松果体和牛视网膜一样，通过免疫组织化学以及 ELISA 和免疫印迹与这些 MAb 发生反应。此外，从兔视网膜中纯化的 S 抗原与牛和豚鼠 S 抗原反应。因此，兔松果体中的 S 抗原与兔视网膜中的 S 抗原不同，也与其他物种的松果体和视网膜中的 S 抗原不同。正如 MAb 与兔松果体中的 S 抗原不发生反应一样，激活的淋巴细胞可能无法识别兔松果体中的 S 抗原，从而不诱导 EAP。