Kalsow C M, Wacker W B
Curr Eye Res. 1986 Aug;5(8):579-86. doi: 10.3109/02713688609015122.
Different forms of experimental autoimmune uveitis (EAU) can be produced by varying protocols to present different autoantigens to several species of experimental animal. We have studied the clinical, histological and serological responses of rabbits to footpad injection of various fractions of retina extract. Rabbits injected with retina extract or S antigen developed posterior uveitis. However, rabbits injected with retina extract, also developed an anterior uveitis and pinealitis not seen in rabbits receiving S antigen. The Serological response of rabbits to retina extract was different than that to purified S antigen. Antisera of rabbits receiving retina extract reacted with rabbit retina and pineal gland as well as with guinea pig retina but not with guinea pig pineal gland. In contrast anti-S antigen sera reacted with rabbit retina and guinea pig retina and pineal gland but not with rabbit pineal gland. Gel filtration chromatography of the ammonium sulfate supernate of retina extract was used to differentiate the antigens with which these two sera reacted. An analysis of these experiments gives preliminary evidence of an autoantigen(s) of rabbit retina and pineal gland that is not S antigen. The existence of multiple autoantigens common to retina and pineal gland in various species is significant in that it further underscores the relationship of these tissues. Furthermore, it is not unrealistic to expect more than one autoantigen of retina or uvea to be involved in autoimmune uveitis.
通过不同的实验方案，向几种实验动物呈现不同的自身抗原，可以诱导出不同形式的实验性自身免疫性葡萄膜炎（EAU）。我们研究了兔子对足垫注射视网膜提取物不同组分的临床、组织学和血清学反应。注射视网膜提取物或S抗原的兔子发生了后葡萄膜炎。然而，注射视网膜提取物的兔子还出现了前葡萄膜炎和松果体炎，而接受S抗原的兔子未出现这些症状。兔子对视网膜提取物的血清学反应与对纯化S抗原的反应不同。接受视网膜提取物的兔子的抗血清与兔视网膜、松果体以及豚鼠视网膜发生反应，但不与豚鼠松果体发生反应。相比之下，抗S抗原血清与兔视网膜、豚鼠视网膜和松果体发生反应，但不与兔松果体发生反应。利用视网膜提取物硫酸铵上清液的凝胶过滤色谱法来区分这两种血清所反应的抗原。对这些实验的分析提供了初步证据，表明兔视网膜和松果体存在一种非S抗原的自身抗原。视网膜和松果体在不同物种中存在多种共同自身抗原，这一点很重要，因为它进一步强调了这些组织之间的关系。此外，预期视网膜或葡萄膜的一种以上自身抗原参与自身免疫性葡萄膜炎并非不切实际。
