College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, People's Republic of China.
J Agric Food Chem. 2012 Aug 22;60(33):8233-47. doi: 10.1021/jf301928r. Epub 2012 Aug 13.
A generic procedure, which involved accelerated solvent extraction and homemade cleanup cartridges, has been developed for the extraction and purification of 35 mycotoxins in various traditional Chinese medicine (TCM) matrixes, i.e., rhizomes and roots, seeds, flowers, and grasses and leaves, for subsequent analysis by ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). All target analytes could be simultaneously quantitated in less than 17 min per run, showing narrow symmetrical peaks. The developed method was also successfully applied in routine monitoring programs, which implied a significant reduction of both effort and time, to investigate the contamination of TCMs. Among 60 commercial TCMs analyzed, 50 were positive. The achieved data underpin the practical application of the UHPLC-MS/MS method as a valuable tool for the trace analysis of multiple mycotoxins in TCMs.
一种通用程序，涉及加速溶剂萃取和自制的净化小柱，已经被开发用于从各种中药（TCM）基质（根茎和根、种子、花、草和叶）中提取和净化 35 种真菌毒素，以便随后进行超高效液相色谱-串联质谱法（UHPLC-MS/MS）分析。所有目标分析物都可以在不到 17 分钟的每个运行时间内同时定量，显示出窄对称的峰。所开发的方法还成功地应用于常规监测计划中，这意味着既节省了精力又节省了时间，从而可以调查 TCM 的污染情况。在所分析的 60 种商业 TCM 中，有 50 种呈阳性。所获得的数据为 UHPLC-MS/MS 方法作为 TCM 中痕量分析多种真菌毒素的有价值工具的实际应用提供了支持。
