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土耳其细粒棘球绦虫马分离株的分子特征分析

Molecular characterization of the horse isolate of Echinococcus granulosus in Turkey.

作者信息

Utuk A E, Simsek S

机构信息

Etlik Veterinary Control Central Research Institute, Parasitology and Bee Diseases Laboratory, 06020 Ankara, Turkey.

出版信息

J Helminthol. 2013 Sep;87(3):305-8. doi: 10.1017/S0022149X12000363. Epub 2012 Jul 24.

Abstract

Cystic echinococcosis is one of the most important helminthozoonoses, affecting various species of intermediate hosts and humans. In this report, we present Echinococcus granulosus infection in a horse and its molecular characterization. Polymerase chain reaction (PCR) amplification of mitochondrial 12S rRNA (mt-12S rRNA) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed. According to the mt-12S rRNA-PCR result, the horse isolate was grouped with E. granulosus sensu stricto (G1-G3) and the partial mt-CO1 sequence corresponded to the G1 strain. This is the first study of the molecular characterization of the horse isolate of E. granulosus in Turkey.

摘要

囊型包虫病是最重要的蠕虫病之一,可感染多种中间宿主及人类。在本报告中,我们介绍了一匹马感染细粒棘球绦虫及其分子特征。进行了线粒体12S核糖体RNA(mt-12S rRNA)的聚合酶链反应(PCR)扩增以及线粒体细胞色素c氧化酶亚基1(mt-CO1)基因的部分测序。根据mt-12S rRNA-PCR结果,该马的分离株与狭义细粒棘球绦虫(G1-G3)归为一组,部分mt-CO1序列与G1株相对应。这是土耳其首次对马源细粒棘球绦虫分离株进行分子特征研究。

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