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土耳其细粒棘球绦虫马分离株的分子特征分析

Molecular characterization of the horse isolate of Echinococcus granulosus in Turkey.

作者信息

Utuk A E, Simsek S

机构信息

Etlik Veterinary Control Central Research Institute, Parasitology and Bee Diseases Laboratory, 06020 Ankara, Turkey.

出版信息

J Helminthol. 2013 Sep;87(3):305-8. doi: 10.1017/S0022149X12000363. Epub 2012 Jul 24.

DOI:10.1017/S0022149X12000363
PMID:22823948
Abstract

Cystic echinococcosis is one of the most important helminthozoonoses, affecting various species of intermediate hosts and humans. In this report, we present Echinococcus granulosus infection in a horse and its molecular characterization. Polymerase chain reaction (PCR) amplification of mitochondrial 12S rRNA (mt-12S rRNA) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed. According to the mt-12S rRNA-PCR result, the horse isolate was grouped with E. granulosus sensu stricto (G1-G3) and the partial mt-CO1 sequence corresponded to the G1 strain. This is the first study of the molecular characterization of the horse isolate of E. granulosus in Turkey.

摘要

囊型包虫病是最重要的蠕虫病之一,可感染多种中间宿主及人类。在本报告中,我们介绍了一匹马感染细粒棘球绦虫及其分子特征。进行了线粒体12S核糖体RNA(mt-12S rRNA)的聚合酶链反应(PCR)扩增以及线粒体细胞色素c氧化酶亚基1(mt-CO1)基因的部分测序。根据mt-12S rRNA-PCR结果,该马的分离株与狭义细粒棘球绦虫(G1-G3)归为一组,部分mt-CO1序列与G1株相对应。这是土耳其首次对马源细粒棘球绦虫分离株进行分子特征研究。

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