Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119 Elazig, Turkey.
Parasitol Res. 2011 Aug;109(2):411-6. doi: 10.1007/s00436-011-2269-8. Epub 2011 Feb 1.
Cystic echinococcosis (CE) due to the formation of a hydatic cyst is a disease commonly seen in humans and animals that can be mortal sometimes. This disease, which is present in many countries around the world, constitutes a great problem for public health and the economy. The aim of this study was to identify Echinococcus granulosus genotypes in formalin-fixed, paraffin-embedded tissues. Tissue samples from 70 human patients with histologically confirmed echinococcosis were analysed by direct PCR of the 12S rRNA gene and by DNA sequence analysis of the CO1 gene of E. granulosus. Of the 70 samples, 29 (41.6%) could be genotypically characterized. Specifically, 26 of 70 were positive by direct G1-3 PCR of the 12S rRNA gene, two of which were identified as G1 by additional CO1 gene sequencing. All the 44 unidentified samples underwent CO1 sequencing, which yielded one G3 and two G6 records, while the remaining 41 samples gave no or inconclusive results. In conclusion, the results from the analysis of human isolates of E. granulosus confirmed the occurrence of G1, G3 and G6 genotypes in Turkey and indicated G1/G3 cluster (E. granulosus sensu stricto) as the predominant genotype.
包虫病（CE）是一种由包虫囊肿形成的疾病，在人类和动物中很常见，有时甚至是致命的。这种疾病在世界上许多国家都存在，对公共卫生和经济构成了巨大的问题。本研究的目的是鉴定福尔马林固定、石蜡包埋组织中的细粒棘球蚴基因型。通过直接 PCR 扩增 12S rRNA 基因和细粒棘球蚴 CO1 基因的 DNA 序列分析，对 70 例经组织学证实的包虫病患者的组织样本进行了分析。在 70 个样本中，有 29 个（41.6%）可以通过 12S rRNA 基因的直接 G1-3 PCR 进行基因分型。其中 26 例经 CO1 基因测序鉴定为 G1，另外 2 例为 G1。所有未鉴定的 44 个样本均进行了 CO1 测序，其中 1 个为 G3，2 个为 G6，而其余 41 个样本则无或结果不确定。总之，对土耳其人类细粒棘球蚴分离株的分析结果证实了 G1、G3 和 G6 基因型的存在，并表明 G1/G3 簇（细粒棘球蚴敏感型）为主要基因型。
