Beato Sílvia, Parreira Ricardo, Calado Manuela, Grácio Maria Amélia A
Instituto de Higiene e Medicina Tropical (IHMT)/Universidade Nova de Lisboa (UNL), Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
Parasitol Int. 2010 Dec;59(4):638-42. doi: 10.1016/j.parint.2010.08.004. Epub 2010 Aug 19.
Infection by the larval stage of the cestode Echinococcus granulosus causes a disease known as cystic echinococcosis or hydatidosis, which is one of the most widespread zoonotic infections of veterinary and medical importance. Numerous studies have shown that E. granulosus exists as a complex of strains differing in a wide variety of criteria. Ten distinct genotypes (G1-G10) have been identified with a potential impact on the pathology, epidemiology and the effect of the measures implemented for the control of hydatidosis. Our main objective was to carry out a preliminary analysis of the genotypes of E. granulosus circulating in the central inland region of Portugal. Parasite samples (hydatid cysts, n=27) were isolated from the liver and lung of sheep and cattle. The DNA extracted from protoscoleces isolated from the fertile cysts served as a template for the PCR amplification of the part of the mitochondrial cytochrome c oxidase subunit 1 (cox1), ATP synthase F0 subunit 6 (atp6) as well as the large (rrnL/16S) and small (rrnS/12S) ribosomal RNA genes. Similarity searches with homologous sequences in the databanks indicated a very high similarity with references assigned to the G1, G3 and/or G1-G3 complex of Echinococcus strains. Phylogenetic analysis (Bayesian approach) supported these observations, and confirmed the assignment of all the analyzed sequences to the G1-G3 genetic cluster.
细粒棘球绦虫幼虫阶段的感染会引发一种名为囊型棘球蚴病或包虫病的疾病，这是兽医和医学领域中最广泛传播且具有重要意义的人畜共患病感染之一。众多研究表明，细粒棘球绦虫以多种标准不同的菌株复合体形式存在。已鉴定出十种不同的基因型（G1 - G10），它们对包虫病的病理学、流行病学以及所实施的控制措施效果具有潜在影响。我们的主要目标是对葡萄牙中部内陆地区传播的细粒棘球绦虫基因型进行初步分析。从绵羊和牛的肝脏及肺中分离出寄生虫样本（包虫囊肿，n = 27）。从可育囊肿中分离出的原头蚴提取的DNA用作模板，用于PCR扩增线粒体细胞色素c氧化酶亚基1（cox1）、ATP合酶F0亚基6（atp6）以及大核糖体RNA基因（rrnL/16S）和小核糖体RNA基因（rrnS/12S）的部分序列。与数据库中同源序列的相似性搜索表明，与细粒棘球绦虫菌株的G1、G3和/或G1 - G3复合体的参考序列具有非常高的相似性。系统发育分析（贝叶斯方法）支持了这些观察结果，并确认所有分析序列都属于G1 - G3基因簇。
