核小体核心在猴病毒40（SV40）DNA的某些富含AT区域的体外优先组装。
Preferential in vitro assembly of nucleosome cores on some AT-rich regions of SV40 DNA.
作者信息
Wasylyk B, Oudet P, Chambon P
出版信息
Nucleic Acids Res. 1979 Oct 10;7(3):705-13. doi: 10.1093/nar/7.3.705.
Abstract
We have found that nucleosomes reconstituted from histone octamers and SV40 DNA Form I by progressively decreasing the salt concentration from 2 M NaCl are formed preferentially around 0.27, 0.37, 0.50 and 0.85 on SV40 DNA (relative to the EcoRI site). When SV40 DNA Form III is used, the nucleosomes form mainly at 0.28, 0.38, 0.61 and 0.83. These sites are very close to both the sites of RNA chain initiation by calf thymus RNA polymerase B on SV40 DNA Form I (0.25, 0.35, 0.42 and 0.88) and the regions of the supercoiled DNA which are readily denaturable by T4 gene 32 protein (0.25, 0.47 and 0.88), and correspond to AT-rich regions as deduced from the nucleotide sequence of SV40 DNA. The physiologically important region around 0.67 is an unfavourable site for all three types of proteins, and corresponds to a GC-rich region surrounding a 17 base pair AT cluster.
摘要
我们发现，通过从2M NaCl逐步降低盐浓度，由组蛋白八聚体和SV40 DNA I型重构的核小体优先在SV40 DNA上相对于EcoRI位点的0.27、0.37、0.50和0.85处形成。当使用SV40 DNA III型时，核小体主要在0.28、0.38、0.61和0.83处形成。这些位点非常接近小牛胸腺RNA聚合酶B在SV40 DNA I型上起始RNA链的位点（0.25、0.35、0.42和0.88）以及超螺旋DNA中易于被T4基因32蛋白变性的区域（0.25、0.47和0.88），并且从SV40 DNA的核苷酸序列推断对应于富含AT的区域。0.67附近的生理重要区域对所有三种类型的蛋白质来说都是不利位点，并且对应于围绕一个17碱基对AT簇的富含GC的区域。
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