Prunell A
EMBO J. 1982;1(2):173-9. doi: 10.1002/j.1460-2075.1982.tb01143.x.
Chromatin was reconstituted from core histones and recombinant plasmid DNAs carrying poly(dA) . poly(dT) inserts of various lengths. A 97-bp insert was found to occupy discrete and regularly-spaced positions on the edges of the nucleosome. This insert cannot, however, be entirely included due to a block in the center of the particle. In contrast, nucleosomes reconstitute on a shorter 20-bp insert. In this case, the insert shows a marked preference for the edges of the particle. Possible structural and physiological implications of these observations are discussed.
染色质由核心组蛋白和携带不同长度聚（dA）·聚（dT）插入片段的重组质粒DNA重构而成。发现一个97碱基对的插入片段占据核小体边缘离散且规则间隔的位置。然而，由于颗粒中心的一个阻断，该插入片段不能被完全包含在内。相比之下，核小体在较短的20碱基对插入片段上重构。在这种情况下，插入片段对颗粒边缘表现出明显的偏好。讨论了这些观察结果可能的结构和生理学意义。
