猴病毒40 DNA上T4基因32蛋白结合位点的定位。
Location of the T4 gene 32 protein binding site on simian virus 40 DNA.
作者信息
Morrow J F, Berg P
出版信息
J Virol. 1973 Dec;12(6):1631-2. doi: 10.1128/JVI.12.6.1631-1632.1973.
Abstract
The T4 gene 32 protein, which binds to single-stranded but not duplex DNA, forms a specifically located denaturation loop in covalently closed circular simian virus 40 (SV40) DNA. Cleavage of the SV40 DNA-gene 32 protein complex with a restriction endonuclease from Hemophilus parainfluenzae shows the loop center to be at 0.46 on the SV40 DNA map. This is within one of the regions of SV40 DNA cleaved preferentially by the single-strand-specific nuclease S(1).
摘要
T4基因32蛋白可与单链DNA结合，而不与双链DNA结合，它在共价闭环猴病毒40（SV40）DNA中形成一个特定位置的变性环。用副流感嗜血杆菌的限制性内切核酸酶切割SV40 DNA - 基因32蛋白复合物，结果显示该环的中心位于SV40 DNA图谱上的0.46处。这位于SV40 DNA被单链特异性核酸酶S(1)优先切割的区域之一内。
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Proc Natl Acad Sci U S A. 1972 Nov;69(11):3448-52. doi: 10.1073/pnas.69.11.3448.
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