Hirosaki University, 3-Bunkyocho, Hirosaki, Aomori 036-8561, Japan.
Arch Virol. 2012 Nov;157(11):2163-9. doi: 10.1007/s00705-012-1419-3. Epub 2012 Jul 24.
As bacteriophages are dependent on the host for multiplication, their infection cycle is expected to be influenced by the host's physiological state. To elucidate how and which steps of the bacteriophage infection cycle are influenced by changes in the physiological state of the host, we quantitatively compared the infection cycle of lytic RNA bacteriophage Qβ in Escherichia coli cultured in rich and minimal media. The adsorption rate constants in both media were almost the same. A difference of 15 min in the latent period and an approximately twofold increase in the rate of phage release were observed, although approximately 10(5) molecules of coat proteins, equivalent to approximately 600-1000 phage particles, accumulated in an infected cell prior to burst. Addition of Mg(2+) to minimal medium markedly affected the Qβ infection cycle, and these results suggest that Mg(2+) is required for the stages of the infectious cycle after adsorption.
由于噬菌体依赖于宿主进行繁殖，因此它们的感染周期预计会受到宿主生理状态的影响。为了阐明噬菌体感染周期是如何以及受到宿主生理状态变化影响的，我们定量比较了在丰富培养基和最低限度培养基中培养的大肠杆菌中裂解 RNA 噬菌体 Qβ的感染周期。两种培养基中的吸附率常数几乎相同。潜伏期相差 15 分钟，噬菌体释放率增加约两倍，尽管在爆发之前，感染细胞中积累了约 10(5)个衣壳蛋白分子，相当于约 600-1000 个噬菌体颗粒。向最低限度培养基中添加 Mg(2+)会显著影响 Qβ感染周期，这些结果表明 Mg(2+)是吸附后感染周期各个阶段所必需的。
