Schoulaker-Schwarz R, Engelberg-Kulka H
J Virol. 1981 Jun;38(3):833-9. doi: 10.1128/JVI.38.3.833-839.1981.
We report here a study on the temperature-sensitive conjugational transfer-deficient mutant Escherichia coli JCFL39, carrying a traD(Ts) mutation, which is also temperature sensitive for group I RNA phages (MS2, f2, and R17). It is shown that, when the mutant was infected with MS2 at 42 degrees C, phage RNA replicated; a 27S MS2 RNA and phage proteins were synthesized. However, neither PFU nor physical MS2 particles were formed, showing that phage assembly was inhibited. In addition, the high temperature affected the membranes of the host mutant: the mutant was hypersensitive to chemicals, and the electrophoretic pattern of the membranal proteins was modified. We suggest that the pleiotropic effects of the traD mutation on MS2 assembly and DNA transfer during conjugation were a result of the changes in the membrane of the mutant.
我们在此报告一项关于温度敏感型接合转移缺陷型突变体大肠杆菌JCFL39的研究，该菌株携带traD(Ts)突变，对I组RNA噬菌体（MS2、f2和R17）也具有温度敏感性。结果表明，当该突变体在42℃下感染MS2时，噬菌体RNA进行复制；合成了27S MS2 RNA和噬菌体蛋白。然而，既未形成噬菌斑单位（PFU），也未形成物理性的MS2颗粒，这表明噬菌体组装受到抑制。此外，高温影响了宿主突变体的膜：该突变体对化学物质高度敏感，膜蛋白的电泳图谱发生了改变。我们认为，traD突变对MS2组装和接合过程中DNA转移的多效性影响是突变体膜变化的结果。
