Programa de Pós-Graduação em Ecologia de Ambientes Aquáticos Continentais, Universidade Estadual de Maringá, Av. Colombo 5790, 87020-900, Maringá, Paraná, Brasil.
J Eukaryot Microbiol. 2014 Jan-Feb;61(1):2-10. doi: 10.1111/jeu.12071. Epub 2013 Sep 7.
Phytotelmata are vegetal structures that hold water from the rain, and organic matter from the forest and the soil, resulting in small, compartmentalized bodies of water, which provide an essential environment for the establishment and development of many organisms. These microenvironments generally harbor endemic species, but many organisms that are found in lakes and rivers, are also present. Here, we report, for the first time, the occurrence of the ciliate genus Paramecium in the tank of the bromeliad species Aechmaea distichantha. The identification of the Paramecium species was performed based on live observations, protargol impregnation, scanning electronic microscopy, and sequencing of the 18s rRNA. The absence of Paramecium from bromeliad tank water was highlighted in several earlier investigations, and may be due to the fact that this species is unable to make cysts. The occurrence of Paramecium multimicronucleatum in our samples may be explained by the proximity between the bromeliads and the river, a potential source of the species. Further, we also believe that the counting methodology used in our study provides a more accurate analysis of the species diversity, since we investigated all samples within a maximum period of 6 h after sampling, allowing minimum loss of specimens.
植物蓄水器是一种植物结构，它可以收集雨水和森林及土壤中的有机物，形成小型分隔的水体，为许多生物的建立和发展提供了必要的环境。这些微环境通常栖息着特有物种，但也有许多在湖泊和河流中发现的生物。在这里，我们首次报道了在凤梨科物种 Aechmaea distichantha 的水槽中发现了草履虫属 Paramecium。Paramecium 物种的鉴定是基于活体观察、蛋白银浸渍、扫描电子显微镜和 18s rRNA 测序进行的。在之前的几项研究中，已经强调了 Paramecium 不存在于凤梨科水槽水中，这可能是因为该物种无法形成胞囊。我们的样本中出现 Paramecium multimicronucleatum 可能是由于凤梨科植物与河流的接近，这是该物种的潜在来源。此外，我们还认为，我们研究中使用的计数方法提供了对物种多样性更准确的分析，因为我们在采样后最长 6 小时内调查了所有样本，从而最大限度地减少了样本的损失。
