均相电化学监测外切酶 III 活性及其在基于靶标循环的核酸检测中的应用。
Homogeneous electrochemical monitoring of exonuclease III activity and its application to nucleic acid testing by target recycling.
机构信息
Laboratoire d'Electrochimie Moléculaire, UMR 7591 CNRS, Université Paris Diderot, Sorbonne Paris Cité, 15 rue Jean-Antoine de Baïf, F-75205 Paris Cedex 13, France.
出版信息
Chem Commun (Camb). 2012 Sep 11;48(70):8772-4. doi: 10.1039/c2cc34511k. Epub 2012 Jul 24.
PMID:22829093
Abstract
A simple and fast electrochemical nucleic acid assay based on a target recycling strategy and the release of a double-stranded DNA intercalating redox probe upon digestion of a specific duplex by exonuclease III is demonstrated.
摘要
基于目标循环策略和外切酶 III 消化特定双链体时双链 DNA 嵌入型氧化还原探针释放的一种简单快速的电化学核酸分析方法得到了验证。
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