Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, Shandong 264003, P.R. China.
Chemistry. 2012 Sep 3;18(36):11343-9. doi: 10.1002/chem.201200671. Epub 2012 Jul 24.
Cy-NiSe and Cy-TfSe were designed and synthesized as sensitive near-infrared (NIR) fluorescent probes for detecting thiols on the basis of Se-N bond cleavage both in cells and in tissues. Since a donor-excited photoinduced electron transfer (d-PET) process occurs between the modulator and the fluorophore, Cy-NiSe and Cy-TfSe have weak fluorescence. On titration with glutathione, the free dye exhibits significant fluorescence enhancement. The two probes are sensitive and selective for thiols over other relevant biological species. They can function rapidly at pH 7.4, and their emission lies in the NIR region. Confocal imaging confirms that Cy-NiSe and Cy-TfSe can be used for detecting thiols in living cells and tissues.
Cy-NiSe 和 Cy-TfSe 被设计和合成作为灵敏的近红外 (NIR) 荧光探针，用于基于 Se-N 键在细胞和组织中裂解来检测巯基。由于调制器和荧光团之间发生供体激发的光致电子转移 (d-PET) 过程，因此 Cy-NiSe 和 Cy-TfSe 的荧光很弱。与谷胱甘肽滴定后，游离染料表现出显著的荧光增强。这两种探针对巯基具有灵敏和选择性，优于其他相关的生物种类。它们在 pH 7.4 下快速起作用，并且它们的发射位于近红外区域。共聚焦成像证实 Cy-NiSe 和 Cy-TfSe 可用于检测活细胞和组织中的巯基。
