Kappel Camila Ripoll, Kretzmann Nélson A, Alvares-da-Silva Mário Reis
Gastroenterology and Hepatology Post-Graduate Course, School of Medicine, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Int J Hepatol. 2012;2012:698905. doi: 10.1155/2012/698905. Epub 2012 Jul 5.
Aims. To determine lymphocyte IRS (IRS1 cells) in HCV patients, correlating it to liver IRS (IRS 1liver) and HOMA-IR. This study tested the hypothesis that IRS1 cells expression can be used as insulin resistance (IR) marker in HCV-infected patients. IRS1 cells were not studied before in HCV infection. Materials and Methods. HCV chronically infected patients, naïve, nonobese, noncirrhotic, and nondiabetic were prospectively included and compared to controls (blood donors). Blood was taken, and leukocytes were separated. IRS1 was determined by real-time PCR. Liver tissue was obtained from transplant donors as controls. Results. 41 HCV-positive patients were included, 26 males (60.5%); mean age of 45 (±7.9); 33 (80.5%) from genotype 1. 6 out of 12 controls were males (50%); mean age was 26.7 (±3.2). There was expression of IRS1 in leukocytes. The median IRS1 cells (HCV) were 0.061 (0.004 to 0.469); the median IRS 1liver (HCV) was 0.0003 (0.00002 to 0.0186)-lower than in controls (resp., P = 0.005 and P = 0.018). HOMA-IR had an inverse correlation with IRS 1liver (P = 0.04). There was no correlation between IRS1 liver and IRS1 cells (P = 0.930). Conclusions. There was expression of IRS1 in leukocytes. IRS1 cells and IRS1 liver were lower in HCV patients than in controls.
目的。确定丙型肝炎病毒（HCV）患者的淋巴细胞胰岛素受体底物（IRS，IRS1细胞），并将其与肝脏胰岛素受体底物（IRS 1肝脏）和稳态模型评估胰岛素抵抗（HOMA-IR）相关联。本研究检验了以下假设：IRS1细胞表达可作为HCV感染患者胰岛素抵抗（IR）的标志物。此前尚未对HCV感染中的IRS1细胞进行研究。材料与方法。前瞻性纳入慢性HCV感染患者，这些患者未接受过治疗、非肥胖、非肝硬化且非糖尿病，并与对照组（献血者）进行比较。采集血液并分离白细胞。通过实时聚合酶链反应（PCR）测定IRS1。从移植供体获取肝脏组织作为对照。结果。纳入41例HCV阳性患者，其中26例男性（60.5%）；平均年龄45岁（±7.9岁）；33例（80.5%）为基因1型。12例对照组中有6例男性（50%）；平均年龄为26.7岁（±3.2岁）。白细胞中存在IRS1表达。IRS1细胞（HCV）的中位数为0.061（0.004至0.469）；IRS 1肝脏（HCV）的中位数为0.0003（0.00002至0.0186），低于对照组（分别为P = 0.005和P = 0.018）。HOMA-IR与IRS 1肝脏呈负相关（P = 0.04）。IRS1肝脏与IRS1细胞之间无相关性（P = 0.930）。结论。白细胞中存在IRS1表达。HCV患者的IRS1细胞和IRS1肝脏低于对照组。
