Department of Chemistry, East China Normal University, Shanghai, PR China.
Analyst. 2012 Sep 21;137(18):4349-55. doi: 10.1039/c2an35473j. Epub 2012 Jul 25.
We report the development of an ultrasensitive amperometric biosensor based on Ag nanoparticles-decorated graphene oxide nanosheets (GO) (Ag-GO) for the rapid detection of clenbuterol (CLB). The morphology and structure of the Ag-GO labeled CLB (Ag-GO-CLB) were characterized by transmission electron microscope (TEM), atomic force microscope (AFM), and ultraviolet-visible spectroscope (UV-vis). The immunosensor was prepared by covalently immobilizing capture antibodies on a multi-walled carbon nanotubes-modified glassy carbon electrode. Through competitive immunoreactions, the Ag-GO-CLB nanocomposites were captured on the immunosensor and the silver was measured by positive differential pulse voltammetry (DPV) in KCl solution for the detection of antigen. The experimental results show a linear response over the range from 0.01 to 10.0 ng mL(-1) with a lower detection limit of 6.8 pg mL(-1) (signal-to-noise ratio of 3). The Ag-GO based immunosensor offers a simple and convenient route for metal-immunoassay labels, which can avoid the complicated and time-consuming dissolving of metal component for ultrasensitive determination. Moreover, the electrochemical immunoassay shows acceptable specificity and stability and is suitable for the determination of CLB in real samples.
我们开发了一种基于 Ag 纳米粒子修饰的氧化石墨烯纳米片（GO）（Ag-GO）的超灵敏安培生物传感器，用于快速检测克伦特罗（CLB）。Ag-GO 标记的 CLB（Ag-GO-CLB）的形态和结构通过透射电子显微镜（TEM）、原子力显微镜（AFM）和紫外可见分光光度计（UV-vis）进行了表征。通过将捕获抗体共价固定在多壁碳纳米管修饰的玻碳电极上，制备了免疫传感器。通过竞争性免疫反应，Ag-GO-CLB 纳米复合材料被捕获在免疫传感器上，并通过在 KCl 溶液中的正差分脉冲伏安法（DPV）测量银，以检测抗原。实验结果表明，在 0.01 至 10.0 ng mL(-1) 的范围内呈线性响应，检测限低至 6.8 pg mL(-1)（信噪比为 3）。基于 Ag-GO 的免疫传感器为金属免疫测定标记提供了一种简单方便的途径，可以避免金属成分溶解的复杂和耗时过程，用于超灵敏测定。此外，电化学免疫分析显示出可接受的特异性和稳定性，适用于实际样品中 CLB 的测定。
