一种用于测试生物膜细菌抗菌敏感性的简单比色法。
A simple colorimetric method for testing antimicrobial susceptibility of biofilmed bacteria.
机构信息
Department of Microbiology, Kyungpook National University, School of Medicine, Daegu 700-422, Republic of Korea.
出版信息
J Microbiol. 2010 Oct;48(5):709-11. doi: 10.1007/s12275-010-0299-z. Epub 2010 Nov 3.
PMID:21046353
Abstract
This study introduces a simple colorimetric method which can measure the antimicrobial susceptibility of bacteria in biofilms using trimethyl tetrazolium chloride (TTC) as an indicator of viable bacteria. The new method was utilized for the evaluation of antibiotic susceptibility of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus biofilms.
摘要
本研究介绍了一种简单的比色法，该方法使用三甲基氯化四氮唑（TTC）作为活细菌的指示剂来测量生物膜中细菌的抗菌药敏性。新方法用于评估大肠杆菌、肺炎克雷伯菌和金黄色葡萄球菌生物膜的抗生素药敏性。
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